碱性单细胞凝胶电泳评价马木居(高本底辐射区)居民淋巴细胞DNA损伤

Q4 Medicine

International Journal of Low Radiation Pub Date : 2017-10-31 DOI:10.1504/IJLR.2017.10008590

Tur Rahardjo, W. Mailana, T. Kisnanto, Darlina, S. Nurhayati, D. Tetriana, D. Ramadhani, M. Syaifudin

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引用次数: 3

摘要

印度尼西亚马穆朱的平均剂量率最高，被认为是印度尼西亚的高本底辐射区。在本研究中，对Mamuju Takandeang村居民进行了一项使用碱性彗星测定法评估DNA损伤的初步研究。从居住在Takandeang村的55名健康献血者和22个对照样本中获得血液样本，并将其加工成测定方法。结果表明，Takandeang村居民的彗尾长度(TL)和彗尾力矩(TM)与对照组差异不显著(p = 0.578, 0.174)。回归分析显示，在对照样本中，DNA损伤随年龄的增长而增加，但相关性不显著(p < 0.05)。相反，在Takandeang居民中观察到显著的负相关(p = 0.02)。这项研究的结果应该在使用更多来自Mamuju的样本的更大规模的研究中得到验证。

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Assessment of DNA damage in lymphocytes of Mamuju (a high background radiation area) inhabitants using alkaline single cell gel electrophoresis

Mamuju in Indonesia has a highest average dose rate and considered as a high background radiation area (HBRA) in Indonesia. Here in this research a preliminary study to evaluate DNA damage using the alkaline comet assay in inhabitants of Takandeang village, Mamuju, was conducted. Blood samples from 55 healthy donors living in Takandeang village and 22 control samples were obtained and processed into the assay. Results showed that there were insignificant differences of comet tail length (TL) and tail moment (TM) between Takandeang village inhabitants and control samples (p = 0.578, 0.174). Regression analysis revealed that the DNA damage was increased with age in control samples, even though the correlation was not significant (p > 0.05). In contrast a significant negative correlation (p = 0.02) was observed in Takandeang inhabitants. Results found in this research should be validating in a larger study using more samples from Mamuju.

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来源期刊

International Journal of Low Radiation Environmental Science-Health, Toxicology and Mutagenesis

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期刊介绍： The IJLR is a peer-reviewed journal dedicated to the publication of research articles, review papers and technical notes in all domains related to low-dose radiation, among which are the biological and health effects in humans and the biota, in vitro and in vivo research on low radiation effects, regulatory and policy aspects, risk estimation and public perception.