New growth medium for culturing lactic acid bacteria as probiotic consortium isolated from fermented fish (Budu)

This study aimed to obtain the best ratio of inoculums and types of alternative media in increasing the growth of the probiotic consortium with the observed variables consisting of viability, cell biomass and decrease in pH. Completely randomized design (CRD) factorial consisting of 2 factors with 3 replications, factor A were the probiotic consortium (A1: Lactobacillus parabuchneri: L. buchneri: L. harbinensis, Schieferilactobacillus harbinensis and Lentilactobacillus parabuchner) with ratio 1:1:1:1:1; A2: same consortium with ratio 1:1:1:1:2; A3: same consortium with ratio 1:1:1:2:1; A4: same consortium with ratio 1:1:2:1:1; A5: same consortium with ratio 1:2:1:1:1; A6: same consortium with ratio 2:1:1:1:1 and factor B were the type of alternative media (B1=control; B2=coconut water (90%) + cassava flour (5%) + fish waste flour (5%); B3=tofu liquid waste (90%) + flour onggok (5%) + fish waste meal (5%); B4= tofu whey (90%) + onggok flour (5%) + fish waste meal (5%). The results showed that there was an interaction between factor A and factor B which was highly significant (P<0.01) on viability, cell biomass and decrease in medium pH. In conclusion, the best ratio of probiotic consortium was 1:1:1:2:1, with growth medium coconut water (90%) + cassava flour (5%) + fish waste flour (5%) which resulted in a viability value of: 3, 02, cell biomass: 22.47 mg/ml and a decrease in the pH of the medium by 2.84.