一种在溶液和纸上检测人类端粒酶活性的全脱氧核糖核酸电路

Zhixue Zhou, Jimmy Gu, J. Brennan, Yingfu Li
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引用次数: 0

摘要

我们设计了一种具有信号放大和信号报告双重功能的简单全dna电路,并将其用于检测人类癌细胞端粒酶活性。该系统利用催化发夹组装(CHA)反应进行扩增,产生分裂的g -四重输出,组装形成完整的鸟嘌呤四重结构作为报告模块。按照设计,线性DNA序列(目标)作为催化剂驱动两个发夹的循环程序化组装,产生具有两个富含鸟嘌呤序列的DNA双工,组装形成完整的Gq结构。Gq元素的形成允许荧光或比色法检测目标。提供了示例来演示癌细胞端粒酶活性在溶液中的荧光检测,以及使用简单的纸装置检测癌细胞端粒酶活性的cha调制比色测定的第一个示例。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
An all-deoxyribonucleic acid circuit for detection of human telomerase activity in solution and on paper
We report on the design of a simple all-DNA circuit with dual functions of signal amplification and signal reporting and its use for detection of human telomerase activity from cancer cells. The system utilizes a catalytic hairpin assembly (CHA) reaction for amplification, which produces split G-quadruplex outputs that assemble to form complete guanine quadruplex structures as reporting modules. As designed, a linear DNA sequence (the target) functions as a catalyst to drive cyclic programmed assembly of two hairpins, producing a DNA duplex with two guanine-rich sequences that assemble to form a complete Gq structure. The formation of the Gq element allows either fluorescence or colorimetric detection of the target. Examples are provided to demonstrate fluorescence detection of cancer cells’ telomerase activities in solution and the first example of a CHA-modulated colorimetric assay for detecting telomerase activities of cancer cells using a simple paper device.
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