基于超高效液相色谱-质谱法比较印度卡纳塔克邦西高止山脉五种Memecylon的抗炎、蛋白酶活性和非靶向代谢产物谱

Bharathi T R
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引用次数: 0

摘要

伞形密梭兰(Memecylon umbellatum)、密梭兰(M. edule)、密梭兰(M. talbotianum)、密梭兰(M. malabaricum)和密梭兰(M. wightii)属于密梭科。该属是著名的传统草药,用于治疗皮肤病。目的是比较五种米梭兰的代谢物组成,并评价其提取物的抗炎和抗蛋白酶活性。采用UPLC-ESI-QTOF-MS分析水/甲醇提取物中的非靶向代谢物,然后用PCA和HCA进行统计分析。通过抑制大豆15-脂氧合酶和蛋白酶测定抗炎和抗蛋白酶活性。利用主成分分析(PCA)对5个种的生育酚、异鼠李素3-葡萄糖苷、异thalic酸、硬脂酰甘油和吡啶进行了鉴定。根据主成分分析,Memecylon属可分为两个类群,M. malabaricum和M. wightii聚在一起,M. umbellatum、M. edule和M. talbotianum聚在一起。粗提物的抗炎活性(抑制大豆15-脂氧合酶)和抗蛋白酶活性(抑制胰蛋白酶和凝血酶)表明,malabaricum和M. talbotianum提取物比其他3种具有更高的抑制作用。这些数据表明,代谢物谱的差异可能与所研究的五种植物提取物的生物活性差异有关。非靶向UPLC-ESI-QTOF-MS技术是一种高效的生物活性成分鉴定方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Comparing Anti-Inflammatory, Anti-protease Activities and Untargeted Metabolite Profiling Based on Ultra-Performance Liquid Chromatography-Mass Spectroscopy of Five Memecylon species from Western Ghats Karnataka, India
Memecylon umbellatum, M. edule, M. talbotianum, M. malabaricum, and M. wightii species belong to the family Melastomataceae. The genus is well-known traditional medicinal herb used to treat skin diseases. The goal is to compare the metabolite makeup of the five Memecylon spp. and to evaluate the extract's anti-inflammatory and antiprotease activities. UPLC-ESI-QTOF-MS was used to profile untargeted metabolites in a water/methanol extract, followed by statistical analysis with PCA and HCA. Anti-inflammatory and antiprotease activity were determined by inhibiting soybean 15-lipoxygenase and Protease. Phenols and flavonoids are the most abundant secondary metabolites in Memecylon spp. Principal component analysis (PCA) was used to identify marker chemicals from five species, tocopherol, isorhamnetin 3-glucoside, isothalic acid, stearoylglycerol, and pyrrolidine. The Memecylon spp. maybe clustered into two groups based on principal component analysis, with M. malabaricum & M. wightii clustered together and M. umbellatum, M. edule & M. talbotianum forming another clustered. The anti-inflammatory (Soybean 15-lipoxygenase inhibition) and antiprotease activities (Trypsin and thrombin Inhibition) of crude extracts suggested that M. malabaricum and M. talbotianum extracts exhibited higher inhibition compared to the other three species. These data suggest that differences in metabolite profiles might be connected to differences in the bioactivity of the five plant extracts examined. The untargeted UPLC-ESI-QTOF-MS technique is efficient for identifying bioactive components of Memecylon spp.
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来源期刊
Bioscience Biotechnology Research Communications
Bioscience Biotechnology Research Communications BIOTECHNOLOGY & APPLIED MICROBIOLOGY-
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