A Commentary on Development of Efficient Enzyme Cocktails for the Bioconversion of Hemicelluloses

The construction of minimum enzymes cocktails could facilitate the bioconversion of specified feedstocks into monosaccharides or XOS. In recent reported researches, the hemicelluloses and recombinant hemicellulases, including endo-s-1,4-xylanases, s-xylosidases, α-L-arabinofuranosidases, acetyl xylan esterase, feruloyl esterase and mannanase as well as their interaction mechanisms were investigated by enzymatic hydrolysis. Four representative interesting works on exploring the synergistic mechanisms and effects between enzymes were reported in this paper. Recombinant enzymes expressed from Pichia pastoris were characterized to reveal the mechanism during the process. This study contributes to the development of efficient enzyme cocktails for the bioconversion of hemicelluloses into monosaccharides and XOS.