WIF1基因的特征及功能分析及其在安哥拉兔毛囊生长发育中的作用

IF 0.8 4区农林科学 Q3 AGRICULTURE, DAIRY & ANIMAL SCIENCE

World Rabbit Science Pub Date : 2022-09-30 DOI:10.4995/wrs.2022.17353

Bohao Zhao, Jiali Li, Xi-Yan Zhang, Zhiyuan Bao, Yang Chen, Xinsheng Wu

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引用次数: 0

摘要

在大多数哺乳动物中，毛囊的生长发育是一个复杂而动态的过程。由于HF生长发育调节兔羊毛产量，探索参与HF生长发育的基因的作用可能是相关的。本研究克隆了安哥拉兔WIF1基因的编码序列。编码区序列长度为1140bp，编码379个氨基酸。生物信息学分析表明，WIF1蛋白不稳定，具有亲水性，位于细胞外区域，含有一种推定的信号肽，在不同哺乳动物中表现出高度同源性。此外，在安哥拉兔组的高羊毛产量中，WIF1显著下调。过表达和敲低研究表明，WIF1调节HF生长发育相关基因和蛋白质，如LEF1和CCND1。WIF1激活β-连环蛋白/TCF的转录活性，促进细胞凋亡，抑制细胞增殖。这些结果表明WIF1可能对HF的发展具有重要意义。因此，本研究为研究WIF1在HF生长发育中的作用提供了理论基础。

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Characterisation and functional analysis of the WIF1 gene and its role in hair follicle growth and development of the Angora rabbit

Growth and development of hair follicles (HF) is a complex and dynamic process in most mammals. As HF growth and development regulate rabbit wool yield, exploring the role of genes involved in HF growth and development may be relevant. In this study, the coding sequence of the Angora rabbit (Oryctolagus cuniculus) WIF1 gene was cloned. The length of the coding region sequence was found to be 1140 bp, which encodes 379 amino acids. Bioinformatics analysis indicated that the WIF1 protein was unstable, hydrophilic and located in the extracellular region, contained a putative signal peptide and exhibited a high homology in different mammals. Moreover, WIF1 was significantly downregulated in the high wool production in the Angora rabbit group. Overexpression and knockdown studies revealed that WIF1 regulates HF growth and development-related genes and proteins, such as LEF1 and CCND1. WIF1 activated β-catenin/TCF transcriptional activity, promoted cell apoptosis and inhibited cellular proliferation. These results indicate that WIF1 might be important for HF development. This study, therefore, provides a theoretical foundation for investigating WIF1 in HF growth and development.

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来源期刊

World Rabbit Science 农林科学-奶制品与动物科学

CiteScore

1.70

自引率

25.00%

发文量

审稿时长

>36 weeks

期刊介绍： World Rabbit Science is the official journal of the World Rabbit Science Association (WRSA). One of the main objectives of the WRSA is to encourage communication and collaboration among individuals and organisations associated with rabbit production and rabbit science in general. Subject areas include breeding, genetics, production, management, environment, health, nutrition, physiology, reproduction, behaviour, welfare, immunology, molecular biology, metabolism, processing and products. World Rabbit Science is the only international peer-reviewed journal included in the ISI Thomson list dedicated to publish original research in the field of rabbit science. Papers or reviews of the literature submitted to World Rabbit Science must not have been published previously in an international refereed scientific journal. Previous presentations at a scientific meeting, field day reports or similar documents can be published in World Rabbit Science, but they will be also subjected to the peer-review process. World Rabbit Science will publish papers of international relevance including original research articles, descriptions of novel techniques, contemporaryreviews and meta-analyses. Short communications will only accepted in special cases where, in the Editor''s judgement, the contents are exceptionally exciting, novel or timely. Proceedings of rabbit scientific meetings and conference reports will be considered for special issues. World Rabbit Science is published in English four times a year in a single volume. Authors may publish in World Rabbit Science regardless of the membership in the World Rabbit Science Association, even if joining the WRSA is encouraged. Views expressed in papers published in World Rabbit Science represent the opinion of the author(s) and do not necessarily reflect the official policy of the WRSA or the Editor-in-Chief.