高等静压法从秘鲁紫玉米芯中提取生物活性物质

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2023-03-22 DOI:10.17268/sci.agropecu.2023.005

Jhoseline Stayce Guillén Sánchez, Cinthia Baú Betim Cazarin, Míriam Regina Canesin, F. G. Reyes, Amadeu Hoshi Iglesias, M. Cristianini

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引用次数: 0

摘要

紫玉米芯是一种农用工业副产品，含有高价值的生物活性化合物，这使其在开发提取工艺方面具有吸引力。本研究的目的是在不同的加工温度下，通过高等静压从紫玉米芯中提取生物活性化合物。使用0.01MPa、250MPa、450MPa和650MPa的压力，持续3分钟，温度分别为25°C、45°C和65°C。将高压萃取与常规萃取（25°C下2.5小时）进行比较。与常规提取2.5小时和65°C不同，在650 MPa和65°C下等静压处理获得的紫玉米芯提取物具有较高的抗氧化活性和生物活性化合物含量（p<0.05），液相色谱法在高等静压（65℃650MPa）和水醇浸渍（65℃2.5h）提取的提取物中鉴定出7种不同的花青素，主要为矢车菊素-3-葡萄糖苷、peargonidin-3-葡萄糖苷和peonidin-3-葡糖苷，以及它们各自的丙二酰衍生物。高等静压使生物活性化合物的提取增加了50%以上，并在更短的时间内获得，从而成为从植物组织中提取生物活性化合物新的替代方法和环保方法。

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Extraction of bioactive compounds from Peruvian purple corn cob by high isostatic pressure

The purple corn cob is an agro-industrial by-product that contains high-value bioactive compounds, which makes its use attractive for the development of extraction processes. The aim of this research was to extract the bioactive compounds from the purple corn cob by high isostatic pressure at different processing temperatures. Pressures of 0.01 MPa, 250 MPa, 450 MPa and 650 MPa for 3 minutes and at temperatures of 25 °C, 45 °C and 65 °C were used. High pressure extraction was compared with conventional extraction (2.5 h at 25°C). The purple corn cob extract obtained by isostatic processing at 650 MPa and 65 °C presented high antioxidant activity and content of bioactive compounds, unlike the conventional extraction of 2.5 h and 65 °C (p < 0.05). However, it did not show a significant difference with the extract obtained at 450 MPa and 45 °C (p > 0.05). Seven different anthocyanins were identified by liquid chromatography in the extracts obtained by high isostatic pressure (650 MPa at 65 °C) and hydroalcoholic maceration (2.5 h at 65 °C), mainly cyanidin-3-glucoside, pelargonidin-3-glucoside and peonidin-3-glucoside, and their respective malonyl derivatives. The high isostatic pressure increased the extraction of bioactive compounds by more than 50% and obtained them in shorter times, thus appearing as a new alternative, and eco-friendly method for the extraction of bioactive compounds from plant tissues.

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.