玉米不同年龄防烟粉虱挥发性化合物分析

Retno Wikan Tyasningsiwi, Witjaksono Witjaksono, S. Indarti
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引用次数: 1

摘要

烟粉虱是红辣椒中的一种害虫,也是秋海棠病毒的载体。这种病媒可以通过综合虫害管理(IPM)措施加以控制。一种潜在的做法是利用玉米作为屏障作物,防止病媒攻击主要作物。本研究的目的是检测从不同年龄的玉米作物中获得的挥发性化合物对烟粉虱的驱避活性。在种植后4周从玉米中收集挥发性化合物(WAP)、6 WAP、8 WAP、10 WAP和12 WAP。用己烷和乙醇两种溶剂设计了挥发性化合物捕获装置,并用气相色谱-质谱法对挥发性化合物进行了分析。用Y型管嗅觉计测定烟粉虱对挥发性物质的排斥性。每种处理采用5次重复。数据采用方差分析法进行95%的分析,并采用最小显著性差异法(LSD)进行进一步分析。观察到的参数是烟粉虱的总量避免了测试的挥发性化合物,直到控制嗅觉计臂的一半。结果表明,各年龄玉米均能产生驱避性化合物,最适年龄为12WAP玉米,驱避率为83.72%。GC-MS分析鉴定出挥发性化合物为香茅、柠檬烯、β-水芹烯、β石竹烯、1.8桉叶素、法尼醇、石竹烯和广藿香醇。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Analysis of Volatile Compound at Different Age of Corn Crops Used as Bemisia tabaci Repellent
Bemisia tabaci is one of the red chili pests that plays a role as a Begomovirus vector. This vector can be controlled through Integrated Pest Management (IPM) practices. One of the potential practices is by utilizing corn as a barrier crop to prevent the vector from attacking the main crop. The aim of this research was to examine the repellence activity of the volatile compound obtained from various ages of corn crop against B. tabaci. The volatile compound was collected from the corns at 4 week after planting (WAP), 6 WAP, 8 WAP, 10 WAP, and 12 WAP. Volatile compound capture device was designed with two solvents, i.e. hexane and ethanol, then the volatile compound analyzed by Gas Chromatography Mass Spectrometry (GC-MS). B. tabaci repellency against volatile compound was examined using Y-tube olfactometer. Each treatment was employed with 5 replications. Data were analyzed using ANOVA with 95% and further analyzed by Least Significant Different (LSD). The parameter observed was the total of B. tabaci avoided the volatile compound tested until half of the control olfactometer arm. The results showed that all ages of the corn produced repellent compound and the most optimal were corn aged 12 WAP with repellency rate of 83.72%. GC-MS analysis identified the volatile compound were citronella, limonene, β-phellandrene, β-caryophyllene, 1.8 cineole, farnesol, caryophyllene, and patchouli alcohol.
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