盐酸屈他韦林和双氯芬酸钾的色谱定量技术研究

IF 0.3 Q4 CHEMISTRY, MULTIDISCIPLINARY

Oriental Journal Of Chemistry Pub Date : 2023-08-30 DOI:10.13005/ojc/390420

Dillip Kumar Dash, A. Sen, S. Saisivam, A. Zanwar, S. Patel, Dhanya B. Sen

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引用次数: 0

摘要

建立了一种简单的反相高效液相色谱法(RP-HPLC)，用于同时测定盐酸氯他弗林(DROTA)和双氯芬酸钾(DICLO)的联合剂型。该方法简便、准确、精密度高。色谱检测采用Phenomenex C18色谱柱(250 mm × 4.6 mm, i.d, 5µm)，流速为1.0 ml/min，流动相为甲醇和水(80:20 v/v, pH 3用邻位磷酸调节)。选择280 nm波长进行紫外检测。DROTA和DICLO的分离用时不到10分钟，分辨率很好，产生的尾矿很少。没有任何辅料的干扰。该方法按照ICH建议进行了验证，准确度、精密度、线性度和系统适应性的标准在每个案例中都是令人满意的。

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Development of a Chromatographic Technique for Quantification of Drotaverine Hydrochloride and Diclofenac Potassium

A straightforward RP-HPLC approach has been developed and validated for the simultaneous estimation of drotaverine hydrochloride (DROTA) and diclofenac potassium (DICLO) in their combined dosage form. This method was found to be simple, accurate, and precise. Chromatographic detection has been carried out on a Phenomenex C18 column (250 mm × 4.6 mm, i.d, 5 µm) with a flow rate of 1.0 ml/min using an isocratic mobile phase consisting of Methanol and Water (80:20 v/v, pH 3 adjusted with ortho phosphoric acid). The wavelength of 280 nm was chosen for the UV detection. The separation of DROTA and DICLO took less than ten minutes, had a good resolution, and produced very little tailing. There was no interference from any excipients. The method was validated in accordance with the ICH recommendations, and the criteria for accuracy, precision, linearity, and system adaptability were all satisfactory in each and every one of the cases.

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来源期刊

Oriental Journal Of Chemistry CHEMISTRY, MULTIDISCIPLINARY-

自引率

20.00%

发文量

172

期刊介绍： Oriental Journal of Chemistry was started in 1985 with the aim to promote chemistry research. The journal consists of articles which are rigorously peer-reviewed. The journal was indexed in Emerging Science citation index in 2016. The Editorial board member consists of eminent international scientist in all fields of Chemistry. Details of each member and their contact information is mentioned in website. The journal has thorough ethics policies and uses plagiarism detection software(ithenticate) to screen each submission. The journal has recently partnered with publons as a part of making our reviews more transparent. The journal has recently incorporated PlumX for article level matrix. The journal is promoting research on all social and academic platforms mentioned in PlumX guidelines. The journal uses google maps to improve on the geographical distribution of Editorial board members as well as authors.