球孢白僵菌和假球孢白僵菌在哈萨克斯坦防治亚洲蝗虫的有效菌株

Q3 Agricultural and Biological Sciences
Ulzhalgas T. Zhumataуeva, Bakhytzhan A. Duissembekov, Yerlan Bozanbaiulu Dutbayev, K. Kidirbayeva, O. A. Alshynbayev, G. A. Bekbulatova
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引用次数: 1

摘要

本研究的目的是对白僵菌属真菌菌株进行遗传鉴定,评价非生物因素对真菌菌落生长、存活及分生孢子产量的影响,并确定以白僵菌为基础的药物半制剂在田间的生物学有效性。采用了生物学和真菌学中公认的传统和现代方法。通过测定TEF区直接核苷酸序列对白僵菌进行鉴定。通过室内试验,考察了菌落生长直径、深耕和浅耕存活率、以球孢白僵菌为原料的药物半制剂类型和暴露时间等因素对药物田间生物效应的影响。在R studio程序中使用P值对统计数据进行处理。昆虫病原真菌在地表培养过程中分生孢子的产量取决于菌株类型和分生孢子的培养时间。白僵菌菌落生长直径受菌种、营养培养基类型、菌落培养温度、菌落培养开始天数等因素的影响。球孢白僵菌在飞蝗翅上的分生孢子存活率与培养温度、分生孢子萌发和培养时间有关。分生白僵菌的产率与菌株类型、底物类型和暴露时间有关。第25天分生孢子继续萌发。真菌在以谷子为基材的基质上生长得更好。在田间,影响迁徙乳杆菌死亡率和生物农药生物药效的因素主要有菌种、生物制剂的喷洒方式和施药时间。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effective Strains of Beauveria Bassiana and B. Pseudobassiana used Against the Asian Locust (Locusta Migratoria L.) in Kazakhstan
: The purpose of the study was to perform genetic identification of the strains of fungi of the Beauveria genus and evaluate the effect of abiotic factors on the growth and survival of colonies and the productivity of conidia of these fungi, as well as to determine the biological effectiveness of the semi-preparative form of the drug based on Beauveria bassiana in the field. Conventional and modern methods accepted in biology and mycology were used. Identification of strains of Beauveria fungi was carried out by determining the direct nucleotide sequence of the TEF region. Laboratory experiments were carried out to evaluate various factors on the diameter of colony growth, survival in deep and surface cultivation, the type of semi-preparative form of the drug based on B. bassiana and the time of its exposure, and the effect on the biological effects of drugs in the field. Statistical data processing was carried out in the R studio program using the P value. The productivity of conidia of entomopathogenic fungi during surface cultivation depended on the type of strain and the duration of cultivation of conidia. The diameter of the growth of Beauveria fungi colonies was influenced by the factors of the strain, the type of nutrient medium, the temperature of colony cultivation, and the number of days after the start of colony cultivation. The survival of conidia of the B. bassiana fungus on the wings of Locusta migratoria L. orrelated with the indicators of cultivation temperature and germination of conidia and cultivation time. The productivity of Beauveria spp. conidia correlate with the type of strain, the type of substrate, and the time of exposure. The conidia continued to germinate on the 25 th day. Fungi grew better on a substrate based on millet. In the field, the mortality rate of L. migratoria L. and the biological effectiveness of biopesticides were influenced by the factors of the strain, the form of spraying with the bio preparation, and the time elapsed after treatment with the biopesticide.
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来源期刊
OnLine Journal of Biological Sciences
OnLine Journal of Biological Sciences Agricultural and Biological Sciences-Agricultural and Biological Sciences (all)
CiteScore
2.10
自引率
0.00%
发文量
35
期刊介绍: :: Cell biology :: Developmental biology :: Structural biology :: Microbiology :: Molecular biology & genetics :: Biochemistry :: Biotechnology :: Biodiversity :: Ecology :: Marine biology :: Plant biology :: Bioinformatics
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