无菌技术

Current Protocols Essential Laboratory Techniques Pub Date : 2019-04-16 DOI:10.1002/cpet.31

Tomasz Bykowski, Jonathan F. Holt, Brian Stevenson

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Aseptic Technique

This article describes common laboratory procedures that can reduce the risk of culture contamination, collectively referred as “aseptic technique.” Two major strategies of aseptic work are described: using a Bunsen burner and a biosafety cabinet. Both methods are presented in the form of general protocols applicable to a variety of laboratory tasks such as pipetting and aliquotting, preparation of growth media, inoculation, passaging, and spreading microorganisms on petri dishes. © 2019 by John Wiley & Sons, Inc.

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Current Protocols Essential Laboratory Techniques

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