伊拉克出生的弗里斯-荷斯坦公牛精子膜、顶体和DNA完整性的月变化影响

Q. Aboud, S. Hatif
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引用次数: 0

摘要

本研究旨在评估一年中几个月对荷斯坦公牛精液质量的影响。在巴格达西部阿布·加雷布人工授精中心进行的一项研究。通过人工阴道从伊拉克出生的15头公牛身上总共采集了160个精液。公牛的年龄在4至5岁之间,研究期为12月至3月和9月。精液样品用Tris碱增量剂稀释。精液按照人工授精中心的程序装在吸管里。评估精液特征(质膜、顶体和DNA完整性)。结果显示,与12月、1月、2月和3月相比,9月的质膜和顶体完整性显著降低(P≤0.01)。与12月、1月、2月和3月相比,9月新鲜精液和冷冻精液的DNA完整性显著下降(P≤0.05)。总之,九月对所有公牛的质膜、顶体和DNA百分比都有负面影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effect of Monthly Variations on the Plasma Membrane, Acrosome and DNA Integrities of Spermatozoa in Friesian Holstein Bulls Born in Iraq
This study was aimed to evaluate the influence of months of the year on the quality of semen in Holstein bulls. A study carried out at artificial insemination centre/ Abou-Ghareeb/ western of Baghdad. A total of 160 ejaculates were collected from 15 bulls born in Iraq via the artificial vagina. The age of the bulls ranged between 4 to 5 years and the study period were December to March and September. The semen samples were diluted with Tris base extender. The semen was packed in a straw according to the program of artificial insemination centre. Semen characteristics (plasma membrane, acrosome, and DNA integrities) were evaluated. The results revealed a significant decrease (P≤0.01) in the plasma membrane and acrosome integrity in September as compared with December, January, February, and March. There was a significant decline (P≤0.05) in DNA integrity in September as compared with December, January, February, and March in fresh and frozen semen. In conclusion, the September month had a negative effect on the plasma membrane, acrosome, and DNA percentage in all bulls.
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