使用CRISPR/Cas9基因敲除筛选功能基因组学的新时代

Journal of molecular and genetic medicine : an international journal of biomedical research Pub Date : 2018-04-09 DOI:10.4172/1747-0862.1000345

W. Cai, Weijing Li, Dan Yang, Hua‐An Xie, Jian Huang

{"title":"使用CRISPR/Cas9基因敲除筛选功能基因组学的新时代","authors":"W. Cai, Weijing Li, Dan Yang, Hua‐An Xie, Jian Huang","doi":"10.4172/1747-0862.1000345","DOIUrl":null,"url":null,"abstract":"In this commentary, we discussed the new exciting progress in CRISPR based screening technology field and highlight recent developments in the area of CRISPR-based functional genomics. High-throughput functional genomics using CRISPR-Cas9 revolutionized our ability to decipher cellular function in health and disease. Despite its limitations, the simplicity and effectiveness of CRISPR/Cas9 based screening, makes an enormous impact on genomic screening and thus scientific discovery.","PeriodicalId":88269,"journal":{"name":"Journal of molecular and genetic medicine : an international journal of biomedical research","volume":" ","pages":"1-3"},"PeriodicalIF":0.0000,"publicationDate":"2018-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4172/1747-0862.1000345","citationCount":"12","resultStr":"{\"title\":\"A New Era in Functional Genomics Using CRISPR/Cas9 Knockout Screening\",\"authors\":\"W. Cai, Weijing Li, Dan Yang, Hua‐An Xie, Jian Huang\",\"doi\":\"10.4172/1747-0862.1000345\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"In this commentary, we discussed the new exciting progress in CRISPR based screening technology field and highlight recent developments in the area of CRISPR-based functional genomics. High-throughput functional genomics using CRISPR-Cas9 revolutionized our ability to decipher cellular function in health and disease. Despite its limitations, the simplicity and effectiveness of CRISPR/Cas9 based screening, makes an enormous impact on genomic screening and thus scientific discovery.\",\"PeriodicalId\":88269,\"journal\":{\"name\":\"Journal of molecular and genetic medicine : an international journal of biomedical research\",\"volume\":\" \",\"pages\":\"1-3\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2018-04-09\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.4172/1747-0862.1000345\",\"citationCount\":\"12\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of molecular and genetic medicine : an international journal of biomedical research\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.4172/1747-0862.1000345\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of molecular and genetic medicine : an international journal of biomedical research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4172/1747-0862.1000345","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 12

摘要

在这篇评论中，我们讨论了基于CRISPR的筛选技术领域令人兴奋的新进展，并重点介绍了基于CRISPR的功能基因组学领域的最新进展。使用CRISPR-Cas9的高通量功能基因组学彻底改变了我们破译健康和疾病中细胞功能的能力。尽管存在局限性，但基于CRISPR/Cas9的筛选的简单性和有效性对基因组筛选和科学发现产生了巨大的影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

A New Era in Functional Genomics Using CRISPR/Cas9 Knockout Screening

In this commentary, we discussed the new exciting progress in CRISPR based screening technology field and highlight recent developments in the area of CRISPR-based functional genomics. High-throughput functional genomics using CRISPR-Cas9 revolutionized our ability to decipher cellular function in health and disease. Despite its limitations, the simplicity and effectiveness of CRISPR/Cas9 based screening, makes an enormous impact on genomic screening and thus scientific discovery.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Journal of molecular and genetic medicine : an international journal of biomedical research

自引率

0.00%

发文量