血斑干法测定乙型肝炎病毒DNA的评价及其在实际条件下的稳定性。

IF 1.6 Q4 INFECTIOUS DISEASES
Rahul Garg, Krithiga Ramachandran, S. Jayashree, Reshu Agarwal, Ekta Gupta
{"title":"血斑干法测定乙型肝炎病毒DNA的评价及其在实际条件下的稳定性。","authors":"Rahul Garg,&nbsp;Krithiga Ramachandran,&nbsp;S. Jayashree,&nbsp;Reshu Agarwal,&nbsp;Ekta Gupta","doi":"10.1016/j.jcvp.2022.100111","DOIUrl":null,"url":null,"abstract":"<div><p>We evaluated the diagnostic performance of dried blood spots (DBS) compared to plasma for detection and quantification of HBV-DNA under real-life conditions. Blood specimens from 100 known cases of chronic hepatitis B (CHB) requested for quantitative HBV-DNA were included. In a subset of 20 patients, three sets of DBS cards were prepared, one kept at -80°C, the other two kept at room temperature for 7 and 14 days, respectively. DBS method demonstrated sensitivity, specificity, and positive predictive value of 95.3%, 100%, and 100%, respectively in comparison to plasma. The mean HBV-DNA load in plasma was 4.8 log10 IU/ml while in DBS was 4.3 log10 IU/ml with a strong correlation (R<sup>2</sup>=0.9087). No significant change in viral load was observed at room temperature for up to 14 days. This study suggests that DBS for HBV viral-load quantitation is a good alternative to plasma as it is stable during storage at room temperature and therefore allows easy handling, storage, and transport of specimens in resource-limited settings.</p></div>","PeriodicalId":73673,"journal":{"name":"Journal of clinical virology plus","volume":"2 4","pages":"Article 100111"},"PeriodicalIF":1.6000,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667038022000503/pdfft?md5=c6f1e3cde94c7563efe14a552e824ac0&pid=1-s2.0-S2667038022000503-main.pdf","citationCount":"2","resultStr":"{\"title\":\"Evaluation of blood samples collected by dried blood spots (DBS) method for hepatitis B virus DNA quantitation and its stability under real life conditions\",\"authors\":\"Rahul Garg,&nbsp;Krithiga Ramachandran,&nbsp;S. Jayashree,&nbsp;Reshu Agarwal,&nbsp;Ekta Gupta\",\"doi\":\"10.1016/j.jcvp.2022.100111\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>We evaluated the diagnostic performance of dried blood spots (DBS) compared to plasma for detection and quantification of HBV-DNA under real-life conditions. Blood specimens from 100 known cases of chronic hepatitis B (CHB) requested for quantitative HBV-DNA were included. In a subset of 20 patients, three sets of DBS cards were prepared, one kept at -80°C, the other two kept at room temperature for 7 and 14 days, respectively. DBS method demonstrated sensitivity, specificity, and positive predictive value of 95.3%, 100%, and 100%, respectively in comparison to plasma. The mean HBV-DNA load in plasma was 4.8 log10 IU/ml while in DBS was 4.3 log10 IU/ml with a strong correlation (R<sup>2</sup>=0.9087). No significant change in viral load was observed at room temperature for up to 14 days. This study suggests that DBS for HBV viral-load quantitation is a good alternative to plasma as it is stable during storage at room temperature and therefore allows easy handling, storage, and transport of specimens in resource-limited settings.</p></div>\",\"PeriodicalId\":73673,\"journal\":{\"name\":\"Journal of clinical virology plus\",\"volume\":\"2 4\",\"pages\":\"Article 100111\"},\"PeriodicalIF\":1.6000,\"publicationDate\":\"2022-11-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S2667038022000503/pdfft?md5=c6f1e3cde94c7563efe14a552e824ac0&pid=1-s2.0-S2667038022000503-main.pdf\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of clinical virology plus\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2667038022000503\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"INFECTIOUS DISEASES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of clinical virology plus","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2667038022000503","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}
引用次数: 2

摘要

我们评估了干燥血斑(DBS)与血浆在现实条件下检测和定量HBV-DNA的诊断性能。来自100例已知慢性乙型肝炎(CHB)病例的血液标本被要求进行HBV-DNA定量分析。在20例患者中,准备了三套DBS卡,一套保存在-80°C,另两套保存在室温下,分别保存7天和14天。与血浆相比,DBS方法的敏感性、特异性和阳性预测值分别为95.3%、100%和100%。血浆中HBV-DNA平均负荷为4.8 log10 IU/ml, DBS中为4.3 log10 IU/ml,相关性较强(R2=0.9087)。在室温下,病毒载量未见显著变化长达14天。该研究表明,DBS用于HBV病毒载量定量是血浆的一种很好的替代方法,因为它在室温下储存时稳定,因此在资源有限的情况下易于处理、储存和运输标本。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Evaluation of blood samples collected by dried blood spots (DBS) method for hepatitis B virus DNA quantitation and its stability under real life conditions

We evaluated the diagnostic performance of dried blood spots (DBS) compared to plasma for detection and quantification of HBV-DNA under real-life conditions. Blood specimens from 100 known cases of chronic hepatitis B (CHB) requested for quantitative HBV-DNA were included. In a subset of 20 patients, three sets of DBS cards were prepared, one kept at -80°C, the other two kept at room temperature for 7 and 14 days, respectively. DBS method demonstrated sensitivity, specificity, and positive predictive value of 95.3%, 100%, and 100%, respectively in comparison to plasma. The mean HBV-DNA load in plasma was 4.8 log10 IU/ml while in DBS was 4.3 log10 IU/ml with a strong correlation (R2=0.9087). No significant change in viral load was observed at room temperature for up to 14 days. This study suggests that DBS for HBV viral-load quantitation is a good alternative to plasma as it is stable during storage at room temperature and therefore allows easy handling, storage, and transport of specimens in resource-limited settings.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Journal of clinical virology plus
Journal of clinical virology plus Infectious Diseases
CiteScore
2.20
自引率
0.00%
发文量
0
审稿时长
66 days
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信