用于真菌研究的CRISPR_Cas系统

IF 5.7 2区生物学 Q1 MYCOLOGY

Fungal Biology Reviews Pub Date : 2020-12-01 DOI:10.1016/j.fbr.2020.10.002

Jean-Paul Ouedraogo, Adrian Tsang

引用次数: 22

摘要

基因组编辑CRISPR-Cas系统，使用Cas9和Cas12a内切酶，提高了我们精确编辑基因组和控制基因表达的能力。我们在此总结利用CRISPR-Cas9和CRISPR-Cas12a在真菌研究中获得的知识。还讨论了限制由CRISPR-Cas基因组编辑引起的脱靶突变发生的策略。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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CRISPR_Cas systems for fungal research

Genome-editing CRISPR-Cas systems, using Cas9 and Cas12a endonucleases, have improved our ability to precisely edit genomes and control gene expression. We summarize here the knowledge gained from using CRISPR-Cas9 and CRISPR-Cas12a in fungal research. Also discussed are strategies developed for limiting the occurrences of off-target mutations caused by CRISPR-Cas genome editing.

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来源期刊

Fungal Biology Reviews MYCOLOGY-

CiteScore

10.60

自引率

0.00%

发文量

期刊介绍： Fungal Biology Reviews is an international reviews journal, owned by the British Mycological Society. Its objective is to provide a forum for high quality review articles within fungal biology. It covers all fields of fungal biology, whether fundamental or applied, including fungal diversity, ecology, evolution, physiology and ecophysiology, biochemistry, genetics and molecular biology, cell biology, interactions (symbiosis, pathogenesis etc), environmental aspects, biotechnology and taxonomy. It considers aspects of all organisms historically or recently recognized as fungi, including lichen-fungi, microsporidia, oomycetes, slime moulds, stramenopiles, and yeasts.