Huwe1失活通过细胞外基质失调促进卵巢癌转移

IF 0.5 4区医学 Q4 OBSTETRICS & GYNECOLOGY

European journal of gynaecological oncology Pub Date : 2022-01-21 DOI:10.31083/j.ejgo4301013

Fujuan Zhang, Jing Guo, Shuaishuai Yu, Xudong Zhao, Yongxin Ma, Dong Yang

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引用次数: 0

摘要

目的:Huwe1对促进包括卵巢癌在内的一系列恶性肿瘤的进展至关重要。但其在卵巢癌转移中的作用尚未见报道。我们研究的目的是评估huwe1与卵巢癌转移之间的相关性。方法:采用流式细胞术对Huwe1小鼠卵巢表面上皮(MOSE)细胞进行鉴定。transwell系统分析细胞迁移和侵袭。通过分析MOSE-Huwe1-CreER和MOSE-Huwe1-Cre细胞RNAseq数据的差异，探讨Huwe1在卵巢癌迁移侵袭中的分子机制。结果:Huwe1基因缺失可显著促进卵巢癌细胞的迁移和侵袭。此外，当接受同种异体移植物的小鼠接受他莫昔芬治疗时，血液中检测到肿瘤细胞的频率更高。转录组分析表明，这种表型可能是由于Huwe1基因敲除引起细胞粘附改变所致。结论:Huwe1失活通过细胞外基质(extracellular matrix, ECM)失调促进卵巢癌转移。

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Huwe1 inactivation promotes ovarian cancer metastasis by extracellular matrix deregulation

Objective: Huwe1 is critical for promoting the progression of a range of malignancies including ovarian cancer. Still, its role in ovarian cancermetastasis has not been reported. The goal of our studywas to evaluate a correlation betweenHuwe1 and ovarian cancer metastasis. Methods: The mouse ovary surface epithelium (MOSE) cells isolated from Huwe1 mice were identified by flow cytometry assay. Cell migration and invasion were analyzed by transwell system. The molecular mechanism of Huwe1 in ovarian cancer migration and invasion was explored by analyzing the difference of RNAseq data between MOSE-Huwe1-CreER and MOSE-Huwe1-Cre cells. Results: Huwe1 deletion significantly promoted tumor migration and invasion in ovarian cancer cells. Moreover, tumor cells were more frequently detected in the blood when the mice bearing allografts were treated with tamoxifen. Transcriptome analysis indicated that this phenotype may be due to the alteration in cell adhesion caused by the Huwe1 knockout. Conclusion: Huwe1 inactivation promoted ovarian cancer metastasis by the extracellular matrix (ECM) deregulation.

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来源期刊

European journal of gynaecological oncology 医学-妇产科学

自引率

25.00%

发文量

审稿时长

1 months

期刊介绍： EJGO is dedicated to publishing editorial articles in the Distinguished Expert Series and original research papers, case reports, letters to the Editor, book reviews, and newsletters. The Journal was founded in 1980 the second gynaecologic oncology hyperspecialization Journal in the world. Its aim is the diffusion of scientific, clinical and practical progress, and knowledge in female neoplastic diseases in an interdisciplinary approach among gynaecologists, oncologists, radiotherapists, surgeons, chemotherapists, pathologists, epidemiologists, and so on.