秋水仙碱诱导藏红花多倍体的研究

IF 1 Q3 AGRONOMY

Journal of Crop Improvement Pub Date : 2021-10-24 DOI:10.1080/15427528.2021.1994502

Mahpara Kashtwari, S. Jan, A. Wani, M. Dhar

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引用次数: 1

摘要

藏红花(Crocus sativus L.)是三倍体(2 n = 3x = 24, x = 8)，这限制了其通过作物育种计划的改进。本研究旨在诱导六倍体，以恢复藏红花的性稳定性。秋水仙碱浓度分别为0.0%、0.025%、0.05%和0.1%，处理球茎3 h、6 h和9 h。共有16个离体芽表现出多倍体化的迹象。与对照(2.8 g)相比，这些胚芽生长缓慢但强劲，形成了大粒(6.0 g)。叶片表皮细胞形态、气孔大小和密度显示出多倍体的特征。根尖细胞学证实为六倍体(2 n = 6x = 48)。六倍体球茎发芽，但不能在田间建立。我们能够开发出一种体外诱导六倍体的方案，但是六倍体球茎在田间的后续生长仍然是恢复藏红花性稳定性的主要挑战。

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Induction of polyploidy in saffron (Crocus sativus L.) using colchicine

ABSTRACT Saffron (Crocus sativus L.) is triploid (2 n = 3x = 24, x = 8), which limits its improvement through crop breeding programs. This study was initiated to induce hexaploidy with the intent of restoring sexual stability in saffron. Corms were treated with colchicine concentrations of 0.0%, 0.025%, 0.05%, and 0.1% for 3-h, 6-h, and 9-h. A total of 16 in-vitro shoots showed signs of polyploidization. These colchiploid shoots showed slow but robust growth and produced large-sized cormlets (6.0 g) compared to the control (2.8 g). Leaf epidermal cell morphology, stomatal size and density showed evidence of polyploidization. Hexaploidy (2 n = 6x = 48) was confirmed by root-tip cytology. The hexaploid corms germinated but could not establish in the field. We were able to develop a protocol for in-vitro induction of hexaploidy in C. sativus, but the subsequent growth of hexaploid corms in the field remains a major challenge in restoring the sexual stability in saffron.

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来源期刊

Journal of Crop Improvement Multiple-

CiteScore

3.30

自引率

7.70%

发文量

期刊介绍： Journal of Crop Science and Biotechnology (JCSB) is a peer-reviewed international journal published four times a year. JCSB publishes novel and advanced original research articles on topics related to the production science of field crops and resource plants, including cropping systems, sustainable agriculture, environmental change, post-harvest management, biodiversity, crop improvement, and recent advances in physiology and molecular biology. Also covered are related subjects in a wide range of sciences such as the ecological and physiological aspects of crop production and genetic, breeding, and biotechnological approaches for crop improvement.