Enhancing of teeth remineralization by electric field aid (in vitro study)

One of the major challenges in teeth re-mineralization is the slow rate of mineralization. In this paper, we developed electric field-aided mineralization system for rapidly regenerating teeth-like structure rapidly. The efficacy of application of electric field combined with Nano-chitosan gel, casein phosphopeptide amorphous calcium phosphate gel and their mixture was investigated as re-mineralizing accelerator for artificially demineralized teeth. Teeth samples have been demineralized by using ethylenediaminetetraacetic acid and subsequently subjected to each gel alone and combined with electric field (20 mA- 5 min). The structural analyses of teeth samples were examined by using scanning electron microscope and X-ray diffraction patterns and its hardness was obtained by Vickers micro-hardness test. The results revealed that the addition of chitosan to casein phosphopeptide amorphous calcium phosphate enhanced the re-mineralization of the tooth structure rather than each one individually. Furthermore, the application of electric field has enhanced teeth hardness and showed highest rate of re-mineralization. In conclusion, the proposed technique could be safely used to reduce the consuming time taken in re-mineralization and efficiently it may be used to increase tooth quality. It is suggested to study other biophysical parameters of using electric fields for in vivo applications.