肝X受体α基因与鸭胴体性状、肉质及脂肪酸组成的相关性

IF 0.3 Q4 AGRICULTURE, DAIRY & ANIMAL SCIENCE
A. Gunawan, N. Azmi, A. Anggraeni
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引用次数: 0

摘要

肝X受体α(LXRα)是一种核受体,在调节参与脂质代谢的基因中起着至关重要的作用。本研究采用聚合酶链式反应-限制性片段长度多态性(PCR-RFLP)技术,对LXRα基因多态性及其与鸭胴体、肉质和脂肪酸组成特征的相关性进行了研究。本研究共使用了98只Cihateup鸭的样本,包括57只雌性和41只12周龄的雄性。产物大小为661bp的扩增子。CC、GC和GG的基因型频率分别为0.21、0.55和0.23。卡方检验表明,LXRα基因外显子2(g.3575C>g)处于Hardy-Weinberg平衡。LXRα基因在g.3575C>g区的SNP与鸭肉品质和脂肪酸含量显著相关(P<0.05)。几个参数有显著影响(P<0.01)。05)对肉质的影响主要表现在胸肉重量、胴体百分比和头百分比上,而相关脂肪酸为饱和脂肪酸(SFA),如栅栏酸(C16:0);γ-和不饱和脂肪酸(UFA),如亚麻酸(C18:3n3);顺式11-二十碳烯酸(C20:1)和11.14-顺式二十碳癸酸(C20:2)。结论LXRα基因可能是选择和生产具有理想不饱和脂肪酸的肉类的遗传标记。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Association of Liver X Receptor Alpha (LXRα) gene related to characteristic of carcass, meat quality and fatty acid composition in ducks
Liver X Receptor Alpha (LXRα) is a nuclear receptor that play a crucial role in regulating of gene involvedin lipid metabolism. The aim of this research was to identify polymorphisms and association of LXRα gene with charateristic of carcass, meat quality and fatty acid composition in ducks using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP). A total sample of 98 Cihateup ducks consisted of 57 females and 41 males with age 12 weeks were used in this study. Product size is 661 bp amplicons. The genotype genes frequencies in CC, GC and GG were 0.21, 0.55 and 0.23 respectively. The chi-square test revealed that LXRα gene (g.3575 C>G) in exon 2 was in Hardy-Weinberg equilibrium. A SNP of LXRα gene in region g.3575 C>G was significantly associated (P<0.05) with duck meat quality and fatty acid content. Several parameters have significant affect (P <0. 05) on meat quality in the breast meat weight, carcass percentage and head percentage, while associated fatty acids were saturated fatty acids (SFA) such as palimitic acid (C16:0); γ-and unsaturated fatty acids (UFA) such as linolenic acid (C18:3n3); cis 11 eicosenoic acid (C20:1) and 11.14 cis-eicosedenoic acid (C20:2). In could be concluded that LXRα gene might be useful as genetic markers to select and produce meat with desirable unsaturated fatty acids.
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来源期刊
Journal of the Indonesian Tropical Animal Agriculture
Journal of the Indonesian Tropical Animal Agriculture AGRICULTURE, DAIRY & ANIMAL SCIENCE-
CiteScore
1.10
自引率
0.00%
发文量
5
审稿时长
8 weeks
期刊介绍: Journal of the Indonesian Tropical Animal Agriculture (JITAA) is a double blind peer-reviewed publication devoted to disseminate all information contributing to the understanding and development of animal agriculture in the tropics by publication of original research papers. The journal covers all aspect relating to Animal Agriculture, including: -Animal breeding and genetics -Animal feeding and nutrition -Animal reproduction -Animal biotechnology -Animal physiology -Animal production and technology -Animal products technology -Animal management and economics -Animal products processing and animal by-products -Animal microbiology -Livestock farming systems -Other related topics in relation to animal science. The topics of research are not only on Indonesian tropical animal agriculture, but also on other tropical regions of the world.
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