小鼠肝炎病毒在实验室小鼠菌落中的分子检测。

Q4 Veterinary

Iranian Journal of Veterinary Science and Technology Pub Date : 2019-02-01 DOI:10.22067/VETERINARY.V11I2.77631

R. Fallahi, F. Abedini, G. Shokri

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引用次数: 1

摘要

动物健康监测需要出具健康证明。病毒性肝炎病毒是小鼠繁殖群体中最重要的传染病之一。本研究采用RT-PCR方法鉴定小鼠肝炎病毒污染。29个标本中有18个被发现感染，患病率为62%。对PCR产物进行纯化和测序。系统发育分析表明，本研究鉴定的菌株与法国报道的一株菌株亲缘关系密切。在常规系统中，不同传染因子的污染是不可避免的，因此最好用干净的动物代替被污染的菌落。

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Molecular detection of mouse hepatitis virus in laboratory mouse colonies.

The animal health monitoring is required to issue health certificates. The viral hepatitis virus is one of the most important infectious agents in mice breeding colonies. This research used RT-PCR to identify contaminations to mouse hepatitis virus. 18 out of 29 specimens were found to be infected, a prevalence of 62%. PCR product was purified and sequenced. Phylogenetic analysis revealed that the identified strain in this study was closely related to a strain reported from France. In the conventional system, contamination with different infectious agents is inevitable, thus it is better to replace the contaminated colonies with clean animals.

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来源期刊

Iranian Journal of Veterinary Science and Technology Veterinary-Veterinary (all)

CiteScore

0.40

自引率

0.00%

发文量

审稿时长

30 weeks