月桂叶乙醇提取物的植物化学筛选及HPTLC分析

IF 1.2 Q4 PHARMACOLOGY & PHARMACY

Journal of Herbs, Spices and Medicinal Plants Pub Date : 2022-09-04 DOI:10.1080/10496475.2022.2117253

A. Khan, Mukesh Kr. Singh, P. Lavhale, R. Kaushik

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引用次数: 1

摘要

摘要对肉桂(Cinnamomum tamala, CT)叶溶剂提取物进行化学成分筛选，并对其类黄酮和酚类物质含量进行测定。采用高效薄层色谱法对槲皮素和丁香酚两种标准标记物进行定量分析。采用氯化铝比色法测定总黄酮和酚类含量，采用福林比色法测定酚类含量。乙醇提取物总黄酮含量最高，分别为141.31±0.053 mg QE g−1和178.96±0.62 mg GAE g−1。采用HPTLC法对槲皮素和丁香酚进行斑点检测(Rf值分别为0.63和0.59)。丁香酚含量为452.6µg，槲皮素含量为705.6µg。

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Phytochemical Screening and HPTLC Analysis of Bio-active Markers of Ethanol Extract of Indian Bay Leaves

ABSTRACT Solvent extracts of Cinnamomum tamala (CT) leaves were screened for phytochemicals, and the flavonoid and phenolic contents were estimated. The two standard marker compounds quercetin and eugenol were also quantified using high-performance thin-layer chromatography (HPTLC). The total flavonoid and phenolic contents were determined using aluminum chloride and Folin–Ciocalteu colorimetric methods, respectively. The ethanolic extracts had the highest total flavonoid and phenolic contents with 141.31 ± 0.053 mg QE g−1 and 178.96 ± 0.62 mg GAE g−1, respectively. The HPTLC method was used to detect spots for quercetin and eugenol (Rf value of 0.63 and 0.59, respectively). The amounts of eugenol and quercetin were 452.6 µg and 705.6 µg in 100 mg of the ethanolic extract of CT leaves, respectively.

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来源期刊

Journal of Herbs, Spices and Medicinal Plants Medicine-Complementary and Alternative Medicine

CiteScore

3.00

自引率

0.00%

发文量

期刊介绍： The Journal of Herbs, Spices & Medicinal Plants is an essential reference filled with recent research and other valuable information associated with herbs, spices, and medicinal plants. The Journal serves as a focus point through which investigators and others may publish material of importance to the production, marketing, and utilization of these plants and associated extracts. The journal covers the following topics: growth, development, horticulture, ecology, physiology, genetics, chemistry, and economics. Original articles, review articles, and book reviews provide information of interest to an international audience of researchers, teachers, technicians, and managers involved with production and/or marketing of herbs, spices, and medicinal plants. Managers of food companies, food processing facilities, medical research laboratories, government agencies, and others interested in new chemicals, food additives, international trade, patents, and other items can easily review new findings. The Journal of Herbs, Spices & Medicinal Plants is a forum in which recent research and other information associated with herbs, spices, and medicinal plants is shared. The Journal represents a centralized database accessible by investigators within the international community that work with or have an interest in herbs, spices, and medicinal plants.