desmoglin 3 (DSG3)作为食管鳞状细胞癌敏感特异性标志物的研究

IF 2 4区 医学 Q3 GASTROENTEROLOGY & HEPATOLOGY
Zhikai Chi, J. Balani, P. Gopal, Suntrea Hammer, C. Lewis, Lan Peng
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引用次数: 1

摘要

尽管P40和P63对常规食管鳞状细胞癌(SCC)的诊断既敏感又特异,但我们最近发现,高分化SCC中P40和P63的免疫反应性明显低于高级别肿瘤。因此,仍然需要一种新的食管鳞状细胞癌标志物,理想的是在高分化鳞状细胞癌中表现更好。我们对结蛋白3(DSG3)在食管鳞状细胞癌、食管腺癌、小细胞肺癌和大B细胞淋巴瘤以及P40和CK5/6中的免疫组织化学进行了表征。世界卫生组织的分类用于将肿瘤分为高分化(WD)、中分化(MD)或低分化(PD)。39例食管鳞状细胞癌中有20个WD、26个MD和17个PD成分。所有食管SCC组分均显示出显著的DSG3免疫反应性(平均80%;范围30%-100%),WD和MD组分中DSG3免疫细胞的比例高于PD组分。没有食管腺癌病例显示出超过10%的DSG3免疫反应性,只有微弱的细胞质染色。在5%的免疫反应性截止值下,DSG3在所有63种SCC成分中的阳性率为100%,在腺癌病例中为18%,在小细胞肺癌或大B细胞淋巴瘤病例中为0%。DSG3的总体特异性为94%。据我们所知,这是首次将DSG3定性为食管SCC的敏感和特异性标志物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Characterization of Desmoglein 3 (DSG3) as a Sensitive and Specific Marker for Esophageal Squamous Cell Carcinoma
Although P40 and P63 are both sensitive and specific for routine esophageal squamous cell carcinoma (SCC) diagnosis, we recently showed that P40 and P63 immunoreactivities were significantly lower in well-differentiated SCC than those in higher grade tumors. Therefore, a novel esophageal SCC marker, ideally performing better in well-differentiated SCC, is still needed. We characterized desmoglein 3 (DSG3) immunohistochemistry in esophageal SCC, esophageal adenocarcinoma, small-cell lung carcinoma, and large B-cell lymphoma, alongside P40 and CK5/6. The World Health Organization classification was used to grade tumors as well-differentiated (WD), moderately differentiated (MD), or poorly differentiated (PD). There were 20 WD, 26 MD, and 17 PD components among 39 esophageal SCC cases. All esophageal SCC components showed significant DSG3 immunoreactivity (mean, 80%; range, 30%–100%), and the proportions of DSG3 immunoreactive cells were higher in the WD and MD components than in the PD components. No esophageal adenocarcinoma cases showed more than 10% DSG3 immunoreactivity with only weak cytoplasmic staining. With a 5% immunoreactivity cutoff, DSG3 positivity was 100% in all 63 SCC components, 18% in adenocarcinoma cases, and 0% in small-cell lung carcinoma or large B-cell lymphoma cases. The overall DSG3 specificity was 94%. To the best of our knowledge, this is the first study to characterize DSG3 as a sensitive and specific marker for esophageal SCC.
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来源期刊
Gastroenterology Research and Practice
Gastroenterology Research and Practice GASTROENTEROLOGY & HEPATOLOGY-
CiteScore
4.40
自引率
0.00%
发文量
91
审稿时长
1 months
期刊介绍: Gastroenterology Research and Practice is a peer-reviewed, Open Access journal which publishes original research articles, review articles and clinical studies based on all areas of gastroenterology, hepatology, pancreas and biliary, and related cancers. The journal welcomes submissions on the physiology, pathophysiology, etiology, diagnosis and therapy of gastrointestinal diseases. The aim of the journal is to provide cutting edge research related to the field of gastroenterology, as well as digestive diseases and disorders. Topics of interest include: Management of pancreatic diseases Third space endoscopy Endoscopic resection Therapeutic endoscopy Therapeutic endosonography.
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