Effects of experimental Trichinella spiralis larvae infection in rats and mice on hematological and biochemical parameters of the host at different infection stages

The purpose of the research is the study of effects of experimental Trichinella spiralis infection in rats and mice on hematological and biochemical blood parameters of the host at different infection stages.Materials and methods. The experiment was performed on mature outbred male rats and male mice that were infected by T. spiralis larvae orally administered at the rate of 10 larvae/1 g of body weight. Blood samples were taken from the rats at 40 days and 3 months after infection; from the mice, on day 40 after infection. The main parameters of peripheral blood in the rats were determined by a MicroCC-20 Plus hematological analyzer (High Technology, Inc. (USA)) using reagents from Clinical Diagnostic Solutions, LLC (Russia). Biochemical blood parameters in the rats and mice were determined by a Clima MC-15 analyzer, RAL Technical el Laboratoria, S.A. (Spain) using reagents produced by Diakon-DS, CJSC (Russia).Results and discussion. At 40 days and 3 months after the rats were experimentally infected with T. spiralis, we observed a consistent increase in the hemoglobin concentration, erythrocytes and leukocytes, and hematocrit, and a decrease in the alkaline phosphatase activity and ALT, and an increase in total protein. On day 40 after infection in the mice, an increase in total protein was observed as in the rats, and a decrease in blood urea. The changes detected in hematological and biochemical parameters in the rats and mice were associated with the toxic effect of Trichinella infection and the response of the host organism. Such changes are dependent on the infection stage and animal species.