岩白菜叶片乙酸乙酯组分中山奈酚的鉴定与定量

Q4 Pharmacology, Toxicology and Pharmaceutics

Journal of Natural Remedies Pub Date : 2023-08-31 DOI:10.18311/jnr/2023/33208

Kinjal Bera, Madhavi Patel

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引用次数: 0

摘要

P.wallichiana DC，通常被称为樟脑草，实际上用于治疗炎症性疾病。采用高分辨液相色谱-质谱法（HRLC-MS）对山奈酚进行了鉴定。采用高效薄层色谱法对山奈酚进行了定量分析。山奈酚的线性范围为200至700纳克/点，相关系数为0.997。该方法的回收率在99.60%至99.85%之间。检测限和定量限分别为25和76.47纳克/点。发现山奈酚在叶片的乙酸乙酯部分中的含量为7.2%。本文首次采用高效液相色谱-质谱联用和高效薄层色谱法对川芎中山奈酚进行了鉴定和定量。

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Identification and Quantification of Kaempferol From Ethyl Acetate Fraction of Pluchea wallichiana DC Leaves

P. wallichiana DC, commonly known as camphor weed, is practically used in treatment of inflammatory disorders. Identification of kaempferol was done using High-Resolution Liquid Chromatography-Mass Spectrometry (HRLC-MS). High-Performance Thin-Layer Chromatography (HPTLC) method was performed and validated for the quantification of kaempferol. With a correlation coefficient of 0.997, the linearity range for kaempferol was 200 to 700 ng/spot. The suggested method had a recovery rate between 99.60 and 99.85 %. Limit of detection and Limit of quantification were determined to be 25 and 76.47 ng per spot, respectively. The amount of kaempferol was found to be 7.2% in ethyl acetate fraction of leaves. This is the first report of identification and quantification of kaempferol from P. wallichiana DC through HRLC-MS and HPTLC methods.

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来源期刊

Journal of Natural Remedies Pharmacology, Toxicology and Pharmaceutics-Pharmacology

CiteScore

0.40

自引率

0.00%

发文量

期刊介绍： Nature can provide all the remedies, for all. All substances produced by nature can be recognized; all cells talk the same language as we all are from the same mother cell. Journal of Natural Remedies is devoted toward the understandings of all remedies from nature. Journal of Natural Remedies publishes original articles related to utilisation of any natural source for remedy including bioactive natural products. Studies related to the resources, chemistry (including analytical), biological activity, biotechnology and pharmacology (including toxicology) of archaea, bacteria, protistas, fungi, plants, animals, viruses and minerals fall within the journal’s scope.