杨边材粉尘的冻融预处理。

IF 0.9 4区农林科学 Q3 MATERIALS SCIENCE, PAPER & WOOD

Wood Research Pub Date : 2020-12-16 DOI:10.37763/wr.1336-4561/65.6.905916

Š. Boháček, A. Pažitný, M. Halaj, J. Balberčák, V. Kuňa

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引用次数: 1

摘要

本文研究了杨边材冷冻和循环冻融预处理的效果，并以含水率为0.7mm的木屑和干燥率为对照进行了试验研究。在酶水解6、24、48、72和96小时后，用15%的酶负载量测量总纤维素含量，测量单糖产量以及乙酸的量。通过“立方”试验，在相同制备的不同重量的样品（31克、25克、62.5克、125克、250克、500克和1000克）上观察了冷冻速率对总产率的影响。为了提高预处理的效率，在-20°C和+25°C的温度下进行了循环冻融实验。结果表明，对杨边材进行一次浸水或干燥冷冻预处理是不够的，因此需要循环冻融来提高单糖的产量。立方体试验分析表明，较慢的冷冻过程对酶的可及性有积极影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Freeze-thaw pretreatment of poplar sapwood dust.

The paper is focused on the effect of freezing and cyclic freezing-thawing pretreatment of poplar sapwood (Populus alba L.). The experimental comparison was carried out by the sawdust fraction 0.7 mm as (a) water-saturated and (b) dry. Monosaccharide yields, as well as an amount of acetic acid, were measured after 6, 24, 48, 72, and 96 hours of enzymatic hydrolysis with 15% load of the enzyme measured to total cellulose content. The influence of freezing rate on total yields was observed on equally prepared samples with different weights (31 g, 25 g, 62.5 g, 125 g, 250 g, 500 g, and 1000 g) by "cubic" tests. To increase the efficiency of pretreatment, a cyclic freezing-thawing experiment at temperatures -20°C and +25°C was performed. The results show that single freezing of grounded poplar sapwood impregnated by water or dry in its matter is not a sufficient pretreatment method, so cyclic freeze-thaw is needed to enhance the yield of monosaccharides. Analysis of cubic test showed that slower freezing process has a positive effect on enzyme accessibility.

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来源期刊

Wood Research 工程技术-材料科学：纸与木材

CiteScore

2.40

自引率

15.40%

发文量

审稿时长

5.4 months

期刊介绍： Wood Research publishes original papers aimed at recent advances in all branches of wood science (biology, chemistry, wood physics and mechanics, mechanical and chemical processing etc.). Submission of the manuscript implies that it has not been published before and it is not under consideration for publication elsewhere.