与非肿瘤膀胱组织相比，尿路上皮膀胱肿瘤中BK多瘤病毒的病毒载量更高

IF 2.1 4区医学 Q3 VIROLOGY

Future Virology Pub Date : 2023-04-14 DOI:10.2217/fvl-2023-0025

H. Kamalinia, Talieh Mostaghimi, Mahdie Taheri, Moein Shirzad, A. A. Pasha, Y. Yahyapour, E. Moudi, Farzin Sadeghi

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引用次数: 0

摘要

目的：本研究检测BK多瘤病毒（BKPyV）基因组和尿路上皮膀胱癌（UBC）及非肿瘤膀胱组织中的病毒载量。材料与方法：采用实时定量聚合酶链式反应测定114份新鲜冷冻膀胱活检样本（61份UBC和53份非肿瘤组织样本）中每个细胞的病毒LT-Ag拷贝数。结果：UBC患者的BKPyV LT-Ag DNA平均载量显著高于无UBC患者。在多变量逻辑回归分析中，BKPyV LT-Ag拷贝数/细胞和吸烟/非法使用药物与癌症相关。受试者操作特征曲线分析确定了0.1拷贝/细胞的膀胱癌症风险。结论：本研究在大多数UBC样本中发现了高BKPyV LT-Ag DNA拷贝数，支持了BKPyV诱导UBC肿瘤发生的假说。

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Higher viral load of BK polyomavirus in urothelial bladder tumors compared with nontumoral bladder tissues

Aim: This study examined BK polyomavirus (BKPyV) genome and viral load in urothelial bladder carcinoma (UBC) and nontumoral bladder tissues. Materials & methods: Quantitative real-time PCR was used to measure viral LT-Ag copy number per cell in 114 fresh-frozen bladder biopsy samples (61 UBC and 53 nontumoral tissue samples). Results: Patients with UBC had a significantly higher mean BKPyV LT-Ag DNA load than those without UBC. In multivariate logistic regression analysis, BKPyV LT-Ag copies/cell and smoking/illicit use of drugs were associated with bladder cancer. Receiver operating characteristic curve analysis identified bladder cancer risk at 0.1 copies/cell. Conclusion: This study found high BKPyV LT-Ag DNA copy numbers in most UBC samples, supporting the hypothesis that BKPyV induces UBC tumorigenesis.

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来源期刊

Future Virology 医学-病毒学

CiteScore

4.00

自引率

3.20%

发文量

审稿时长

6-12 weeks

期刊介绍： Future Virology is a peer-reviewed journal that delivers essential information in concise, at-a-glance article formats. Key advances in the field are reported and analyzed by international experts, providing an authoritative but accessible forum for this ever-expanding area of research. It is an interdisciplinary forum for all scientists working in the field today.