泰国一名利什曼病患者马提尼克利什曼原虫基因组组装及基因组注释

IF 1.4 Q3 PARASITOLOGY

Journal of Parasitology Research Pub Date : 2022-03-22 DOI:10.1155/2022/8768574

Songtham Anuntakarun, A. Phumee, Vorthon Sawaswong, K. Praianantathavorn, Witthaya Poomipak, R. Jitvaropas, P. Siriyasatien, S. Payungporn

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引用次数: 1

摘要

利什曼病是由利什曼原虫引起的一种寄生虫病，分布于世界各地。据报道，本地利什曼病是由泰国马提尼克利什曼原虫感染引起的。该物种在培养中分离并进行高通量全基因组测序。总计30.8 对整个基因组36条染色体中的Mb进行了组装、注释和鉴定。研究中的马提尼氏乳杆菌被证明是分离到同一个分支中的，因此与先前鉴定的马提尼氏乳杆菌（LU_Lmar_1.0）密切相关，这是通过对其基因组序列以及代表性动植物种的基因组序列的系统发育分析确定的。全基因组开放阅读框的总数预测了8209个蛋白质编码基因，其中359个是推定的毒力因子，包括两个先前已知的，例如半胱氨酸蛋白酶C和超氧化物歧化酶B1。该研究结果将有助于进一步注释和与其他马提尼利什曼原虫的比较。

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Genome Assembly and Genome Annotation of Leishmania martiniquensis Isolated from a Leishmaniasis Patient in Thailand

Leishmaniasis is a parasitic disease caused by Leishmania spp. with worldwide distribution. Autochthonous leishmaniasis has been reported to result from the infection by Leishmania martiniquensis in Thailand. This species was isolated in culture and subjected to high-throughput whole-genome sequencing. A total of 30.8 Mb in 36 chromosomes of the whole genome was assembled, annotated, and characterized. The L. martiniquensis under study was shown to segregate into the same clade and thus closely related to the previously identified L. martiniquensis (LU_Lmar_1.0), as determined by phylogenetic analysis of their genomic sequences along with those of representative kinetoplastid species. The total number of open reading frames genomewide predicts 8,209 protein-coding genes, of which 359 are putative virulence factors, including two previously known, e.g., cysteine proteinase C and superoxide dismutase B1. The results obtained from this study will be useful for further annotation and comparison with other Leishmania martiniquensis in the future.

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来源期刊

Journal of Parasitology Research Medicine-Infectious Diseases

CiteScore

3.50

自引率

9.10%

发文量

审稿时长

13 weeks

期刊介绍： Journal of Parasitology Research is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies in all areas of basic and applied parasitology. Articles covering host-parasite relationships and parasitic diseases will be considered, as well as studies on disease vectors. Articles highlighting social and economic issues around the impact of parasites are also encouraged. As an international, Open Access publication, Journal of Parasitology Research aims to foster learning and collaboration between countries and communities.