麦地子甲醇叶提取物对6-羟基多巴胺（6-OHDA）诱导PC12细胞毒性的抗氧化和神经保护作用

IF 2.8 4区医学 Q2 TOXICOLOGY

Toxicology Mechanisms and Methods Pub Date : 2017-01-08 DOI:10.1080/15376516.2016.1275908

Ángel Rodríguez-Huamán, Sandra Casimiro-Gonzales, Jorge Antonio Chávez-Pérez, Carla Gonzales-Arimborgo, Richard Cisneros-Fernández, Luis Ángel Aguilar-Mendoza, G. Gonzales

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引用次数: 23

摘要

摘要活性氧（ROS）通常在细胞代谢过程中产生，有强有力的证据表明，过量产生的ROS会损害细胞，并可能与各种神经退行性疾病有关。本研究旨在研究美叶蛇床子（MACA）甲醇叶提取物对暴露于6-羟基多巴胺（6-OHDA）的PC12细胞的神经毒性的影响。对“maca”叶片的新鲜样品进行处理，以获得叶片提取物，并通过测定抗氧化能力、细胞活力和对PC12细胞的细胞毒性测定，评估6-OHDA对PC12的细胞毒性作用下的PC12细胞神经生物学活性。抗氧化活性的测试结果显示，甲醇和水性组分的最大值分别为2262.37和1305.36，以Trolox当量（TEAC）表示。剂量为10的细胞活力测定 μg提取物在6和12时分别增加了31%和60% 预处理h。在相同剂量和暴露时间下的细胞毒性测定显示乳酸脱氢酶（LDH）活性降低31.4%和47.8%，超氧化物歧化酶（SOD）活性增加。该结果使我们能够确认，“maca”的甲醇叶提取物在体外具有抗氧化保护、提高细胞活力和降低对6-OHDA产生的氧化应激的细胞毒性的神经生物学活性。总之，本研究表明，梅叶提取物通过抗氧化作用对6-OHDA诱导的毒性具有保护作用。

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Antioxidant and neuroprotector effect of Lepidium meyenii (maca) methanol leaf extract against 6-hydroxy dopamine (6-OHDA)-induced toxicity in PC12 cells

Abstract Reactive oxygen species (ROS) are normally produced during cell metabolism, there is strong evidence to suggest that ROS produced in excess impair the cell and may be etiologically related to various neurodegenerative diseases. This study was undertaken to examine the effects of Lepidium meyenii (MACA) methanol leaf extract on neurotoxicity in PC12 cell exposed to 6-hydroxydopamine (6-OHDA). Fresh samples of “maca” leaves were processed in order to obtain foliar extracts and to evaluate the neurobiological activity on PC12 cells, subjected to the cytotoxic effect of 6-OHDA through the determination of the capacity antioxidant, cell viability and cytotoxicity assays on PC12 cells. The results of the tests of antioxidant activity, showed maximum values of 2262.37 and 1305.36 expressed in Trolox equivalents (TEAC), for the methanolic and aqueous fractions respectively. Cell viability assays at a dose of 10 μg extract showed an increase of 31% and 60% at 6 and 12 h of pretreatment, respectively. Cytotoxicity assays at the same dose and exposure time showed a 31.4% and 47.8% reduction in lactate dehydrogenase (LDH) activity and an increase in superoxide dismutase (SOD) activity. The results allow us to affirm that the methanolic foliar extract of “maca” presents in vitro neurobiological activity of antioxidant protection, increase in cell viability and reduction of cytotoxicity against oxidative stress generated by 6-OHDA. In conclusion, the present study shows a protective role for Lepidium meyenii leaf extract on 6-OHDA-induced toxicity by an antioxidant effect.

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来源期刊

Toxicology Mechanisms and Methods TOXICOLOGY-

自引率

3.10%

发文量

期刊介绍： Toxicology Mechanisms and Methods is a peer-reviewed journal whose aim is twofold. Firstly, the journal contains original research on subjects dealing with the mechanisms by which foreign chemicals cause toxic tissue injury. Chemical substances of interest include industrial compounds, environmental pollutants, hazardous wastes, drugs, pesticides, and chemical warfare agents. The scope of the journal spans from molecular and cellular mechanisms of action to the consideration of mechanistic evidence in establishing regulatory policy. Secondly, the journal addresses aspects of the development, validation, and application of new and existing laboratory methods, techniques, and equipment. A variety of research methods are discussed, including: In vivo studies with standard and alternative species In vitro studies and alternative methodologies Molecular, biochemical, and cellular techniques Pharmacokinetics and pharmacodynamics Mathematical modeling and computer programs Forensic analyses Risk assessment Data collection and analysis.