Sonika Sharma, S. Datta, S. Chatterjee, M. G. Vairale, R. Prasad, Bidisha Das, Vanlalhmuaka .
{"title":"废水中Lytic噬菌体的分离鉴定及其在减毒中的应用","authors":"Sonika Sharma, S. Datta, S. Chatterjee, M. G. Vairale, R. Prasad, Bidisha Das, Vanlalhmuaka .","doi":"10.14429/dlsj.5.15599","DOIUrl":null,"url":null,"abstract":"Present work wasperformedto isolate, enrich and characterizebacteriophageusing basic laboratory set-up, and to demonstrate their lytic potential in pathogen reduction. Following standard microbiological and molecular biological procedures, we examined cultivable bacteria and bacteriophages in wastewater sample. Subsequently, from cultures of wastewater sample, we isolated and characterized bacterial isolate, which was thenusedtoenrichlytic bacteriophages, using a combination of double layer plaque-assay, isolation and genetic techniques to isolate bacteriophage against Pseudomonas aeruginosa. Subsequently decontamination capability of this phage was assessed in form of spray and matrix adsorbed formulations. Storage stability of phagepreparations at different temperatures was also studied by calculating reduction in phage titer. Presentstudies demonstratelytic potential of phageas an eco-friendly alternative to conventional control approachesagainstbacterial contamination.","PeriodicalId":36557,"journal":{"name":"Defence Life Science Journal","volume":" ","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2020-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Isolation and Characterization of Lytic Bacteriophages from Wastewater and its Application in Pathogen Reduction\",\"authors\":\"Sonika Sharma, S. Datta, S. Chatterjee, M. G. Vairale, R. Prasad, Bidisha Das, Vanlalhmuaka .\",\"doi\":\"10.14429/dlsj.5.15599\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Present work wasperformedto isolate, enrich and characterizebacteriophageusing basic laboratory set-up, and to demonstrate their lytic potential in pathogen reduction. Following standard microbiological and molecular biological procedures, we examined cultivable bacteria and bacteriophages in wastewater sample. Subsequently, from cultures of wastewater sample, we isolated and characterized bacterial isolate, which was thenusedtoenrichlytic bacteriophages, using a combination of double layer plaque-assay, isolation and genetic techniques to isolate bacteriophage against Pseudomonas aeruginosa. Subsequently decontamination capability of this phage was assessed in form of spray and matrix adsorbed formulations. Storage stability of phagepreparations at different temperatures was also studied by calculating reduction in phage titer. Presentstudies demonstratelytic potential of phageas an eco-friendly alternative to conventional control approachesagainstbacterial contamination.\",\"PeriodicalId\":36557,\"journal\":{\"name\":\"Defence Life Science Journal\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2020-04-08\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Defence Life Science Journal\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.14429/dlsj.5.15599\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"Pharmacology, Toxicology and Pharmaceutics\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Defence Life Science Journal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.14429/dlsj.5.15599","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Pharmacology, Toxicology and Pharmaceutics","Score":null,"Total":0}
Isolation and Characterization of Lytic Bacteriophages from Wastewater and its Application in Pathogen Reduction
Present work wasperformedto isolate, enrich and characterizebacteriophageusing basic laboratory set-up, and to demonstrate their lytic potential in pathogen reduction. Following standard microbiological and molecular biological procedures, we examined cultivable bacteria and bacteriophages in wastewater sample. Subsequently, from cultures of wastewater sample, we isolated and characterized bacterial isolate, which was thenusedtoenrichlytic bacteriophages, using a combination of double layer plaque-assay, isolation and genetic techniques to isolate bacteriophage against Pseudomonas aeruginosa. Subsequently decontamination capability of this phage was assessed in form of spray and matrix adsorbed formulations. Storage stability of phagepreparations at different temperatures was also studied by calculating reduction in phage titer. Presentstudies demonstratelytic potential of phageas an eco-friendly alternative to conventional control approachesagainstbacterial contamination.