E. Gomes, U.G. Paula Lana, Marina Eleutério Gói De Moraes Rodrigues, S. M. de Sousa, C. Oliveira, Amanda Nayê GUIMARÃES TAVARES
{"title":"玉米芽孢杆菌菌株多相特性研究","authors":"E. Gomes, U.G. Paula Lana, Marina Eleutério Gói De Moraes Rodrigues, S. M. de Sousa, C. Oliveira, Amanda Nayê GUIMARÃES TAVARES","doi":"10.18512/rbms2020v19e1190","DOIUrl":null,"url":null,"abstract":"The use of bioinoculants with plant growth-promoting bacteria (PGPB) is a sustainable agricultural practice that has been expanding worldwide. Thus, it is fundamental to characterize these bacteria molecularly to provide better security and traceability for bioinoculants production. This work aimed to identify, characterize and develop specific molecular markers for Bacillus strains related to plant-growing promotion. Five strains were identified as B. pumillus (B32), B. thuringiensis (B116), B. megaterium (B119) and B. subitilis (B2082 and B2084) using 16S rRNA gene sequencing and MALDI-TOF mass spectrometry. Repetitive element palindromic (Rep-PCR) and amplified ribosomal DNA restriction analysis (ARDRA) techniques allowed the discrimination of the strains, except B2082 and B2084 that presented identical genetic profiles, indicating that they are same or genetically close isolates. Moreover, specific molecular markers were developed for B116 and B119 strains and may be used for microbial inoculants production quality control, strains traceability and to detail PGPB colonization.","PeriodicalId":34859,"journal":{"name":"Revista Brasileira de Milho e Sorgo","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2020-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"3","resultStr":"{\"title\":\"POLYPHASIC CHARACTERIZATION OF Bacillus STRAINS ISOLATED FROM MAIZE\",\"authors\":\"E. Gomes, U.G. Paula Lana, Marina Eleutério Gói De Moraes Rodrigues, S. M. de Sousa, C. Oliveira, Amanda Nayê GUIMARÃES TAVARES\",\"doi\":\"10.18512/rbms2020v19e1190\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The use of bioinoculants with plant growth-promoting bacteria (PGPB) is a sustainable agricultural practice that has been expanding worldwide. Thus, it is fundamental to characterize these bacteria molecularly to provide better security and traceability for bioinoculants production. This work aimed to identify, characterize and develop specific molecular markers for Bacillus strains related to plant-growing promotion. Five strains were identified as B. pumillus (B32), B. thuringiensis (B116), B. megaterium (B119) and B. subitilis (B2082 and B2084) using 16S rRNA gene sequencing and MALDI-TOF mass spectrometry. Repetitive element palindromic (Rep-PCR) and amplified ribosomal DNA restriction analysis (ARDRA) techniques allowed the discrimination of the strains, except B2082 and B2084 that presented identical genetic profiles, indicating that they are same or genetically close isolates. Moreover, specific molecular markers were developed for B116 and B119 strains and may be used for microbial inoculants production quality control, strains traceability and to detail PGPB colonization.\",\"PeriodicalId\":34859,\"journal\":{\"name\":\"Revista Brasileira de Milho e Sorgo\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2020-11-16\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"3\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Revista Brasileira de Milho e Sorgo\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.18512/rbms2020v19e1190\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Revista Brasileira de Milho e Sorgo","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.18512/rbms2020v19e1190","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
POLYPHASIC CHARACTERIZATION OF Bacillus STRAINS ISOLATED FROM MAIZE
The use of bioinoculants with plant growth-promoting bacteria (PGPB) is a sustainable agricultural practice that has been expanding worldwide. Thus, it is fundamental to characterize these bacteria molecularly to provide better security and traceability for bioinoculants production. This work aimed to identify, characterize and develop specific molecular markers for Bacillus strains related to plant-growing promotion. Five strains were identified as B. pumillus (B32), B. thuringiensis (B116), B. megaterium (B119) and B. subitilis (B2082 and B2084) using 16S rRNA gene sequencing and MALDI-TOF mass spectrometry. Repetitive element palindromic (Rep-PCR) and amplified ribosomal DNA restriction analysis (ARDRA) techniques allowed the discrimination of the strains, except B2082 and B2084 that presented identical genetic profiles, indicating that they are same or genetically close isolates. Moreover, specific molecular markers were developed for B116 and B119 strains and may be used for microbial inoculants production quality control, strains traceability and to detail PGPB colonization.
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