登嘉楼部分农场无刺蜂(Kelulut)蜂蜜病原菌群的分离和鉴定

IF 0.6 Q3 AGRICULTURE, MULTIDISCIPLINARY
Noor Aimi Shazana Mohd Yusoff, Fisal Ahmad, A. Zamri, S. B. Abdul Razak, Muhammad Fauzi Mahmud, Tuan Zainazor Tuan Chilek
{"title":"登嘉楼部分农场无刺蜂(Kelulut)蜂蜜病原菌群的分离和鉴定","authors":"Noor Aimi Shazana Mohd Yusoff, Fisal Ahmad, A. Zamri, S. B. Abdul Razak, Muhammad Fauzi Mahmud, Tuan Zainazor Tuan Chilek","doi":"10.47836/pjtas.46.3.08","DOIUrl":null,"url":null,"abstract":"In Malaysia, stingless bees can be categorised into two genera: Melipona and Trigona, known as “kelulut”. The high demand for kelulut honey boosts the production of the honey industry. Previous studies reported that stingless bee (kelulut) honey and its products were contaminated with pathogenic bacteria during harvesting and processing. This research aims to isolate and identify the pathogenic bacteria in kelulut honey. Forty-eight samples of kelulut honey (open and closed pot) and propolis were obtained from selected farms in Terengganu by focusing on a major stingless bee species available in Malaysia, Heterotrigona itama. In addition, the swabbing technique was done on the wooden beehive of the kelulut to evaluate the environmental contamination. The pathogenic bacteria were isolated using specifically selected agar, such as Bacillus cereus agar (for B. cereus), Baird-Parker agar (for Staphylococcus aureus), and MacConkey agar (for other pathogenic bacteria), which were confirmed through a biochemical test. All samples were analysed, and the results showed that B. cereus (7/48), Pseudomonas aeruginosa (10/48), Pantoea spp. (11/48), Serratia plymuthica (6/48), and S. aureus (9/48) were obtained in the samples. This study indicates that kelulut honey was contaminated with B. cereus, P. aeruginosa, Pantoea spp., S. plymuthica, and S. aureus. Isolated pathogenic bacteria may exist in the kelulut honey through food handlers, utensils, and the environment. Hence, the stakeholders should strictly follow good standard operating procedures and guidelines by the kelulut honey industry to prevent foodborne illness.","PeriodicalId":19890,"journal":{"name":"Pertanika Journal of Tropical Agricultural Science","volume":" ","pages":""},"PeriodicalIF":0.6000,"publicationDate":"2023-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Pathogenic Bacterial Communities Isolated and Identified in Stingless Bee (Kelulut) Honey from Selected Farms in Terengganu\",\"authors\":\"Noor Aimi Shazana Mohd Yusoff, Fisal Ahmad, A. Zamri, S. B. Abdul Razak, Muhammad Fauzi Mahmud, Tuan Zainazor Tuan Chilek\",\"doi\":\"10.47836/pjtas.46.3.08\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"In Malaysia, stingless bees can be categorised into two genera: Melipona and Trigona, known as “kelulut”. The high demand for kelulut honey boosts the production of the honey industry. Previous studies reported that stingless bee (kelulut) honey and its products were contaminated with pathogenic bacteria during harvesting and processing. This research aims to isolate and identify the pathogenic bacteria in kelulut honey. Forty-eight samples of kelulut honey (open and closed pot) and propolis were obtained from selected farms in Terengganu by focusing on a major stingless bee species available in Malaysia, Heterotrigona itama. In addition, the swabbing technique was done on the wooden beehive of the kelulut to evaluate the environmental contamination. The pathogenic bacteria were isolated using specifically selected agar, such as Bacillus cereus agar (for B. cereus), Baird-Parker agar (for Staphylococcus aureus), and MacConkey agar (for other pathogenic bacteria), which were confirmed through a biochemical test. All samples were analysed, and the results showed that B. cereus (7/48), Pseudomonas aeruginosa (10/48), Pantoea spp. (11/48), Serratia plymuthica (6/48), and S. aureus (9/48) were obtained in the samples. This study indicates that kelulut honey was contaminated with B. cereus, P. aeruginosa, Pantoea spp., S. plymuthica, and S. aureus. Isolated pathogenic bacteria may exist in the kelulut honey through food handlers, utensils, and the environment. Hence, the stakeholders should strictly follow good standard operating procedures and guidelines by the kelulut honey industry to prevent foodborne illness.\",\"PeriodicalId\":19890,\"journal\":{\"name\":\"Pertanika Journal of Tropical Agricultural Science\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":0.6000,\"publicationDate\":\"2023-07-18\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Pertanika Journal of Tropical Agricultural Science\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.47836/pjtas.46.3.08\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"AGRICULTURE, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Pertanika Journal of Tropical Agricultural Science","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.47836/pjtas.46.3.08","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"AGRICULTURE, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0

摘要

在马来西亚,无刺蜜蜂可以分为两个属:Melipona和Trigona,被称为“kelulut”。对克鲁鲁蜂蜜的高需求促进了蜂蜜行业的生产。先前的研究报告称,无刺蜂蜂蜜及其产品在收获和加工过程中被病原菌污染。本研究旨在分离鉴定克鲁鲁特蜂蜜中的致病菌。48份kelulut蜂蜜(开放式和封闭式罐)和蜂胶样本是从丁加奴的选定农场获得的,重点是马来西亚的一种主要无刺蜜蜂,异三角蜂。此外,还对克鲁鲁特的木蜂窝进行了擦拭技术,以评估其对环境的污染。使用特别选择的琼脂分离致病菌,如蜡样芽孢杆菌琼脂(用于蜡样芽孢)、Baird-Parker琼脂(用于金黄色葡萄球菌)和MacConkey琼脂(用于其他致病菌),这些琼脂通过生化测试得到确认。对所有样品进行了分析,结果显示,样品中获得了蜡样芽孢杆菌(7/48)、铜绿假单胞菌(10/48)、Pantea spp.(11/48)、plymuthica沙雷氏菌(6/48)和金黄色葡萄球菌(9/48)。本研究表明,克鲁鲁特蜂蜜被蜡样芽孢杆菌、铜绿假单胞菌、Pantea spp.、plymuthica和金黄色葡萄球菌污染。分离的致病菌可能通过食品处理人员、器具和环境存在于克鲁鲁特蜂蜜中。因此,利益相关者应严格遵守kelulut蜂蜜行业良好的标准操作程序和指南,以预防食源性疾病。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Pathogenic Bacterial Communities Isolated and Identified in Stingless Bee (Kelulut) Honey from Selected Farms in Terengganu
In Malaysia, stingless bees can be categorised into two genera: Melipona and Trigona, known as “kelulut”. The high demand for kelulut honey boosts the production of the honey industry. Previous studies reported that stingless bee (kelulut) honey and its products were contaminated with pathogenic bacteria during harvesting and processing. This research aims to isolate and identify the pathogenic bacteria in kelulut honey. Forty-eight samples of kelulut honey (open and closed pot) and propolis were obtained from selected farms in Terengganu by focusing on a major stingless bee species available in Malaysia, Heterotrigona itama. In addition, the swabbing technique was done on the wooden beehive of the kelulut to evaluate the environmental contamination. The pathogenic bacteria were isolated using specifically selected agar, such as Bacillus cereus agar (for B. cereus), Baird-Parker agar (for Staphylococcus aureus), and MacConkey agar (for other pathogenic bacteria), which were confirmed through a biochemical test. All samples were analysed, and the results showed that B. cereus (7/48), Pseudomonas aeruginosa (10/48), Pantoea spp. (11/48), Serratia plymuthica (6/48), and S. aureus (9/48) were obtained in the samples. This study indicates that kelulut honey was contaminated with B. cereus, P. aeruginosa, Pantoea spp., S. plymuthica, and S. aureus. Isolated pathogenic bacteria may exist in the kelulut honey through food handlers, utensils, and the environment. Hence, the stakeholders should strictly follow good standard operating procedures and guidelines by the kelulut honey industry to prevent foodborne illness.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Pertanika Journal of Tropical Agricultural Science
Pertanika Journal of Tropical Agricultural Science Agricultural and Biological Sciences-Agronomy and Crop Science
CiteScore
1.10
自引率
16.70%
发文量
64
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信