Detection and quantification of anionic detergent in milk using rhodamine 6gdye and toluene solvent

In the present study different proportion of synthetic milk (2, 4, 8 10 and 15 %) was blended with natural milk and tested for detergent qualitatively as well as quantitatively successfully using Rhodamine 6G dye and Toluene solvent. In positive milk samples, the colour of the solvent changed from transparent to pink, and the intensity of colour increased with an increase in detergent concentration. In contrast, the colour of the solvent layer in the control milk sample remained transparent. Quantity of detergent determined by uptake of benzethonium chloride. This new method was sensitive enough to detect as low as 16 mg of detergent per 100 ml of adulterated milk.