纳米银间接暴露对哺乳大鼠肝脏和脑组织caspase-8和caspase-9水平的影响

IF 1.4 Q4 NANOSCIENCE & NANOTECHNOLOGY
M. Fatemi
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引用次数: 1

摘要

目的:纳米银(AgNp)对成年动物模型的不良健康影响已被充分记录。然而,关于AgNp对敏感发育阶段的毒性作用的数据很少。本研究旨在研究母乳暴露于AgNp对大鼠后代肝脏和大脑细胞凋亡诱导的影响。材料和方法:哺乳期Wistar大鼠灌胃暴露于载体(去离子水)或两剂AgNp(25和100mg/kg)21天。在出生后第21天从母亲的雄性幼崽身上采集肝脏和大脑样本。分别使用ICP-MS分析和ELISA测定组织中的银含量以及胱天蛋白酶-8和胱天蛋白酶-9的水平。对于组织病理学检查,使用苏木精-伊红(H&E)染色对组织切片进行染色,并通过光学显微镜进行检查。结果:暴露于AgNp的幼崽和母乳的肝脏和大脑中的银含量显著增加,呈剂量依赖性。此外,暴露于高剂量AgNp(100mg/kg-1)的幼崽的肝脏和大脑中胱天蛋白酶-9的水平显著增加,而与对照组相比,实验组的胱天蛋白酶-8水平没有观察到显著变化。组织病理学研究还表明,暴露于高剂量AgNp的幼崽的肝脏和大脑中存在组织损伤。结论:哺乳期暴露于AgNp可通过内源性途径诱导大鼠后代组织凋亡。然而,为了记录这些发现,还需要进一步调查。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The effects of indirect exposure of nanosilver on caspase-8 and caspase-9 levels in liver and brain of suckling rats
Objective(s): The adverse health effects of nanosilver (AgNp) on adult animal models have been well documented. However, data is scarce regarding the toxic effects of AgNp on sensitive developmental stages. The present study aimed to investigate the effects of maternal milk exposure to AgNp on apoptosis induction in the liver and brain of the offspring of rats. Materials and Methods: Lactating Wistar rats were intragastrically exposed to the vehicle (deionized water) or two doses of AgNp (25 and 100 mg/kg) for 21 days. Liver and brain samples were collected from the male pups of the mothers on postnatal day 21. The silver content and levels of caspase-8 and caspase-9 in the tissues were measured using the ICP-MS analysis and ELISA assay, respectively. For histopathological examinations, the tissue sections were stained using the hematoxylin-eosin (H&E) stain and examined by light microscopy.Results: A significant, dose-dependent increase was observed in the silver content of the liver and brain of the pups and maternal milk exposed to AgNp. In addition, the level of caspase-9 significantly increased in the liver and brain in the pups exposed to the high dose of AgNp (100 mg/kg-1), while no significant changes were observed in the level of caspase-8 in the experimental groups compared to the controls. Histopathological studies also demonstrated tissue damage in the liver and brain of the pups exposed to the high dose of AgNp. Conclusion: According to the results, lactational exposure to AgNp may induce apoptosis via the intrinsic pathway in the offspring tissues of rats. However, further investigation is required in order to document these findings.
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来源期刊
Nanomedicine Journal
Nanomedicine Journal NANOSCIENCE & NANOTECHNOLOGY-
CiteScore
3.40
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12 weeks
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