{"title":"家鸡卵巢基质金属蛋白酶16 mRNA表达及调控","authors":"Dominika Wolak, A. Hrabia","doi":"10.3409/fb_70-4.17","DOIUrl":null,"url":null,"abstract":"In mammals, membrane-bound matrix metalloproteinases (MT-MMPs) are thought to play an important role in ovarian remodeling. However, the role and regulation of these proteases in the ovary of birds remain largely unknown. One of MT-MMPs, i. e., MMP-16, has been found in the hen ovary;\n therefore, this study was undertaken to examine whether the transcript level of MMP-16 changes during follicle development and whether gonadotropins and estrogen are involved in the regulation of this enzyme expression. The relative expression of MMP-16 mRNA in the ovarian follicles (white,\n yellowish, small yellow, and the granulosa and theca layers of three of the largest yellow preovulatory [F3-F1]) was examined 22 h and 3 h before F1 follicle ovulation as well as following equine chorionic gonadotropin (eCG) or tamoxifen (estrogen receptor modulator, TMX) treatments by quantitative\n real-time polymerase chain reaction (qRT-PCR). MMP-16 transcripts were detected in all examined ovarian tissues of control and treated hens. The relative expression of MMP-16 depended on follicular size/maturation and the layer of the follicular wall. A relatively higher expression of MMP-16\n mRNA in the granulosa layer at 3 h compared to 22 h before ovulation of F1 was found. The injections of eCG decreased transcript abundance of MMP-16 in white and small yellow follicles, as well as in the theca layer of F3-F2 and the granulosa layer of the F1 follicle. In turn, TMX caused an\n increase in mRNA expression of MMP-16 in the theca layer of the largest preovulatory follicles and a decrease in the granulosa layer of the F1 follicle. Our results provide the first mRNA expression analysis of MMP-16 in the hen ovary under different physiological states. In addition, results\n indicate a possible role of gonadotropins and estrogen in regulating the transcription of MMP-16 in the chicken ovary.","PeriodicalId":50438,"journal":{"name":"Folia Biologica-Krakow","volume":" ","pages":""},"PeriodicalIF":0.8000,"publicationDate":"2022-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Ovarian mRNA Expression and Regulation of Matrix Metalloproteinase 16 in the Domestic Hen\",\"authors\":\"Dominika Wolak, A. Hrabia\",\"doi\":\"10.3409/fb_70-4.17\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"In mammals, membrane-bound matrix metalloproteinases (MT-MMPs) are thought to play an important role in ovarian remodeling. However, the role and regulation of these proteases in the ovary of birds remain largely unknown. One of MT-MMPs, i. e., MMP-16, has been found in the hen ovary;\\n therefore, this study was undertaken to examine whether the transcript level of MMP-16 changes during follicle development and whether gonadotropins and estrogen are involved in the regulation of this enzyme expression. The relative expression of MMP-16 mRNA in the ovarian follicles (white,\\n yellowish, small yellow, and the granulosa and theca layers of three of the largest yellow preovulatory [F3-F1]) was examined 22 h and 3 h before F1 follicle ovulation as well as following equine chorionic gonadotropin (eCG) or tamoxifen (estrogen receptor modulator, TMX) treatments by quantitative\\n real-time polymerase chain reaction (qRT-PCR). MMP-16 transcripts were detected in all examined ovarian tissues of control and treated hens. The relative expression of MMP-16 depended on follicular size/maturation and the layer of the follicular wall. A relatively higher expression of MMP-16\\n mRNA in the granulosa layer at 3 h compared to 22 h before ovulation of F1 was found. The injections of eCG decreased transcript abundance of MMP-16 in white and small yellow follicles, as well as in the theca layer of F3-F2 and the granulosa layer of the F1 follicle. In turn, TMX caused an\\n increase in mRNA expression of MMP-16 in the theca layer of the largest preovulatory follicles and a decrease in the granulosa layer of the F1 follicle. Our results provide the first mRNA expression analysis of MMP-16 in the hen ovary under different physiological states. In addition, results\\n indicate a possible role of gonadotropins and estrogen in regulating the transcription of MMP-16 in the chicken ovary.\",\"PeriodicalId\":50438,\"journal\":{\"name\":\"Folia Biologica-Krakow\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":0.8000,\"publicationDate\":\"2022-12-06\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Folia Biologica-Krakow\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.3409/fb_70-4.17\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Folia Biologica-Krakow","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.3409/fb_70-4.17","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOLOGY","Score":null,"Total":0}
Ovarian mRNA Expression and Regulation of Matrix Metalloproteinase 16 in the Domestic Hen
In mammals, membrane-bound matrix metalloproteinases (MT-MMPs) are thought to play an important role in ovarian remodeling. However, the role and regulation of these proteases in the ovary of birds remain largely unknown. One of MT-MMPs, i. e., MMP-16, has been found in the hen ovary;
therefore, this study was undertaken to examine whether the transcript level of MMP-16 changes during follicle development and whether gonadotropins and estrogen are involved in the regulation of this enzyme expression. The relative expression of MMP-16 mRNA in the ovarian follicles (white,
yellowish, small yellow, and the granulosa and theca layers of three of the largest yellow preovulatory [F3-F1]) was examined 22 h and 3 h before F1 follicle ovulation as well as following equine chorionic gonadotropin (eCG) or tamoxifen (estrogen receptor modulator, TMX) treatments by quantitative
real-time polymerase chain reaction (qRT-PCR). MMP-16 transcripts were detected in all examined ovarian tissues of control and treated hens. The relative expression of MMP-16 depended on follicular size/maturation and the layer of the follicular wall. A relatively higher expression of MMP-16
mRNA in the granulosa layer at 3 h compared to 22 h before ovulation of F1 was found. The injections of eCG decreased transcript abundance of MMP-16 in white and small yellow follicles, as well as in the theca layer of F3-F2 and the granulosa layer of the F1 follicle. In turn, TMX caused an
increase in mRNA expression of MMP-16 in the theca layer of the largest preovulatory follicles and a decrease in the granulosa layer of the F1 follicle. Our results provide the first mRNA expression analysis of MMP-16 in the hen ovary under different physiological states. In addition, results
indicate a possible role of gonadotropins and estrogen in regulating the transcription of MMP-16 in the chicken ovary.
期刊介绍:
Folia Biologica (Kraków) is an international online open access journal accepting original scientific articles on various aspects of zoology: phylogeny, genetics, chromosomal studies, ecology, biogeography, experimental zoology and ultrastructural studies. The language of publication is English, articles are assembled in four issues per year.