FLOTAC基本技术是一种新的根结线虫(Meloidogyne spp.)提取方法

A. Troccoli, G. d’Errico, T. D’Addabbo, N. Sasanelli, A. Bosco, M. P. Maurelli, L. Rinaldi, G. Cringoli
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引用次数: 0

摘要

FLOTAC技术已被广泛认为是提取人类和动物寄生虫的有效方法。本研究首次应用FLOTAC基本技术从土壤和植物根中提取植物寄生线虫。采用FBT法和离心浮选法、浸根法等常规线虫提取方法,分别从受害土壤和番茄根中提取根结线虫根结线虫卵和二期幼虫(J2)。与离心浮选法提取相比,FBT从土壤中提取的隐孢子虫J2和卵的数量总是显著增加,平均277个卵和J2 mL-1土壤。相反,在从番茄根中提取鸡蛋和J2的过程中,FBT和根浸渍技术之间没有观察到显著差异。结果表明,FBT对植物寄生线虫的提取也非常有效。由于其准确性和敏感性,FBT似乎特别适合在广阔的地理区域进行线虫调查,在那里需要准确快速地检测目前的植物寄生线虫。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The FLOTAC basic technique as a new extraction method for root-knot nematodes (Meloidogyne spp.) from soil and roots
FLOTAC Techniques have been widely acknowledged as an effective method for the extraction of human and animal parasites. The present study is the first application of FLOTAC basic technique (FBT) for the extraction of phytoparasitic nematodes from soil and infested plant roots. Eggs and second stage juveniles (J2) of the root-knot nematode Meloidogyne incognita were extracted from infested soil and tomato roots either by FBT and conventional nematode extraction methods, such as centrifugal flotation and root maceration techniques, respectively. The number of M. incognita J2 and eggs extracted from soil by FBT was always significantly higher compared to the extraction with the centrifugal flotation method, averaging 277 vs 35 eggs and J2 mL-1 soil. Conversely, no significant differences were observed between FBT and the root maceration technique in the extraction of eggs and J2 from tomato roots. Results demonstrated that FBT can be highly effective also for the extraction of phytoparasitic nematodes. Due to its accuracy and sensitivity, FBT seems particularly suitable for nematode surveys in wide geographical areas, where an accurate and rapid detection of present phytoparasitic nematofauna is required.
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