年轻春葡萄藤中反式白藜芦醇、反式ε-葡萄苷和反式δ-葡萄苷的含量——样品干燥的影响

IF 1 Q3 HORTICULTURE

Horticultural Science Pub Date : 2022-09-30 DOI:10.17221/19/2021-hortsci

N. Vrchotová, J. Tříska, R. Střálková, Štěpán Horník, J. Sýkora, J. Balík, I. Soural, Lukáš Toupal, R. Sotolář

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引用次数: 0

摘要

主要研究了样品制备方法对春葡萄中反式白藜芦醇、反式ε-葡萄苷和反式δ-葡萄苷含量的影响。对六个葡萄品种的样品进行了分析。（“Hibernal”、“Malverina”、“Kolor”、“Fioletovij Augustovskij”、“Grüner Veltliner”和“Blaufränkisch”）在室温下冷冻干燥或缓慢干燥（三个月），使用DAD和FLD检测器以及LC-MS通过HPLC进行提取和分析。通过与用漆酶二聚化反式白藜芦醇制备的反式-δ-长春花苷的比较，证实了反式-Δ-长春花甙的存在，并通过LC-NMR验证了其结构。样品在室温下的缓慢干燥使得在所有研究的V品种中都能合成大量的二苯乙烯。与冷冻干燥的样品相比，葡萄藤导致更高的二苯乙烯含量。本研究的结果表明了在提取和分析之前干燥甘蔗样品的方法的重要性。

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Content of trans-resveratrol, trans-ε-viniferin and trans-δ-viniferin in young spring grapevine canes – the influence of samples drying

The main objective was to study the effect of sample preparation on the content of trans-resveratrol, trans-ε-viniferin and trans-δ-viniferin in young spring grapevine canes. The samples of six varieties of Vitis vinifera L. (‘Hibernal’, ‘Malverina’, ‘Kolor’, ‘Fioletovij Augustovskij’, ‘Grüner Veltliner’ and ‘Blaufränkisch’) were either lyophilised or slow dried at room temperature (three months), extracted and analysed by HPLC using DAD and FLD detectors and by LC-MS. The presence of trans-δ-viniferin was confirmed by comparison with trans-δ-viniferin prepared by dimerization of trans-resveratrol using laccase and its structure was verified by LC-NMR. The slow drying of the samples at room temperature enables the synthesis of a significant amount of stilbenes in all the studied varieties of V. vinifera canes resulting in a higher stilbene content compared to the lyophilised samples. The results of this study show the importance of the method of drying the cane sample before the extraction and analysis.

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来源期刊

Horticultural Science Horticulture-园艺

CiteScore

2.20

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： The journal publishes results of basic and applied research from all areas of horticulture, fruit-growing, vegetable-growing, wine-making and viticulture, floriculture, ornamental gardening, garden and landscape architecture, concerning plants that are grown under the conditions of European temperate zone, or field plants that are considered as horticultural cultures. Original scientific papers, short communications and review articles are published in the journal. Papers are published in English (British spelling).