ZERUMBONE的抗血管生成活性和ROS-介导的肺癌细胞系损伤

Q4 Pharmacology, Toxicology and Pharmaceutics

Ankara Universitesi Eczacilik Fakultesi Dergisi Pub Date : 2023-07-04 DOI:10.33483/jfpau.1112778

Shaymaa Fadhel ABBAS ALBAAYİT, M. K. Mohammed

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引用次数: 0

摘要

目的:Zerumbone (ZER)是一种众所周知的具有抗癌作用的天然化合物。因此，本研究探讨了ZER在非小细胞肺癌NCI-H460细胞系中抑制胸苷磷酸化酶(TP)的潜力和触发活性氧(ROS)介导的细胞毒性的能力。材料与方法:采用胸苷磷酸化酶抑制试验评价其抗血管生成活性。流式细胞术检测DCFDA染色后活性氧(ROS)的生成。结果与讨论:ZER对TP有较强的抑制作用，IC50值为50.3±0.31 μg/ml或230±1.42µM。经ZER处理的NCI-H460细胞产生了55.7%的显著ROS。因此，ZER在肺癌细胞中发挥抗血管生成特性，调节ROS的产生，为癌症药物提供更好的治疗指标。

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ANTIANGIOGENIC ACTIVITY AND ROS-MEDIATED LUNG CANCER CELL LINE INJURY OF ZERUMBONE

Objective: Zerumbone (ZER) is a well-known natural compound that has been reported to have anti-cancer effect. Thus, this study investigated the ZER potential to inhibit Thymidine Phosphorylase (TP) and the ability to trigger Reactive oxygen species (ROS)-mediated cytotoxicity in non-small cell lung cancer, NCI-H460, cell line. Material and Method: The antiangiogenic activity for ZER was evaluated by using the thymidine phosphorylase inhibitory test. Reactive oxygen species (ROS) production was determined via DCFDA dye by using flow cytometry. Result and Discussion: ZER was found to be potent TP inhibitory with the IC50 value of 50.3± 0.31 μg/ml or 230±1.42 µM. NCI-H460 cells upon treatment with ZER produced significant ROS by 55.7%. Consequently, ZER exerts anti-angiogenic properties and modulates ROS production in lung cancer cells, serving as leads for better therapeutic index in cancer drug.

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来源期刊

Ankara Universitesi Eczacilik Fakultesi Dergisi Pharmacology, Toxicology and Pharmaceutics-Pharmaceutical Science

CiteScore

0.80

自引率

0.00%

发文量