迷迭香酸单独或与鼠李糖乳杆菌联合可改善虹鳟的生长、免疫、抗氧化防御和肝功能对氨胁迫的抗性

IF 1.8 4区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE
S. Hajirezaee, M. Khanjani
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引用次数: 1

摘要

摘要将迷迭香酸(RS)和鼠李糖乳杆菌(LR)单独或组合添加到虹鳟(Oncorhynchus mykiss)日粮中,以测试它们对氨胁迫的保护作用。将鱼(31.4±0.6g)随机分为六组,每组三个重复,如下:T1:基础食物作为对照,T2:浓度为1.5×108CFU/g的LR,T3:浓度为3×108CFu/g的LR、T4:1g RS/kg、T5:3g RS/kg、T6:1.5×108cfu/g LR+1 g RS/kg和T7:3×108 CFU/g LR+3 g RS/kg。喂食60天后,鱼类暴露在氨胁迫下。喂食期后,与对照组相比,补充的鱼的最终体重(FW)、增重(WG)和比生长率(SGR)最高,饲料转化率(FCR)最低(P<0.05),与对照组相比,添加1.5×108CFU/g LR+1g RS/kg和1.5×108cfu/g LR的鱼的蛋白酶和脂肪酶活性显著升高(P<0.05)。通常,添加日粮的鱼,特别是T2和T6组,溶菌酶、替代补体活性(ACH50)、总Ig、硝基蓝四氮唑试验(NBT)、髓过氧化物酶(MPO),补体组分3(C3)、补体组分4(C4)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GPx)。另一方面,与对照组相比,T2和T6组的丙二醛(MDA)、葡萄糖和皮质醇浓度以及丙氨酸氨基转移酶(ALT)、碱性磷酸酶(ALP)和乳酸脱氢酶(LDH)水平最低(P<0.05),溶菌酶、ACH50、总Ig、NBT、MPO、C3、C4、SOD、CAT、GPx均显著高于对照组(P<0.05),MDA、葡萄糖、皮质醇、ALT、ALP、LDH均较对照组低(P<0.01),RS和鼠李糖乳杆菌的联合施用有效地改善了生长性能和健康状况,并增强了虹鳟对氨毒性的抵抗力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Rosmarinic acid alone or in combination with Lactobacillus rhamnosus ameliorated resistance to ammonia stress in the rainbow trout, Oncorhynchus mykiss: growth, immunity, antioxidant defense and liver functions
Abstract Rosmarinic acid (RS) and Lactobacillus rhamnosus (LR) were added singularly or in combination to rainbow trout (Oncorhynchus mykiss) diets to test their efficacy in the protection against ammonia stress. Fish (31.4±0.6 g) were randomly allocated to six groups in three replicates, as follows: T1: basic food as control, T2: LR with a concentration of 1.5 × 108 CFU /g, T3: LR with a concentration of 3 × 108 CFU/g, T4: 1 g RS/kg, T5: 3 g RS/kg, and T6: 1.5 × 108 CFU/g LR + 1 g RS/kg and T7: 3 × 108 CFU/g LR + 3 g RS/kg. After 60 days feeding, fish were exposed to ammonia stress. After the feeding period, the supplemented fish had the highest final body weight (FW), weight gain (WG), and specific growth rate (SGR), and the lowest feed conversion ratio (FCR) as compared with the control group (P<0.05). Amylase, protease and lipase activities were noticed markedly higher in fish supplemented with 1.5 × 108 CFU/g LR + 1 g RS/kg and 1.5 × 108 CFU/g LR diets compared to the control (P<0.05). Generally, fish in supplemented diets, particularly T2 and T6 groups, had the highest lysozyme, alternative complement activity (ACH50), total Ig, nitroblue tetrazolium test (NBT), myeloperoxidase (MPO), complement component 3 (C3), complement component 4 (C4), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx). On the other hand, T2 and T6 groups had the lowest malondialdehyde (MDA), glucose, and cortisol concentrations as well as alanine aminotransferase (ALT), alkaline phosphatase (ALP), lactate dehydrogenase (LDH) enzyme levels when compared with the control (P<0.05). After ammonia stress, fish in the supplemented groups, particularly T2 and T6, generally showed significantly higher values of lysozyme, ACH50, total Ig, NBT, MPO, C3, C4, SOD, CAT, GPx and lower levels of MDA, glucose, cortisol, ALT, ALP, LDH when compared with the control (P<0.05). In conclusion, a combined administration of RS and L. rhamnosus effectively improved growth performance and health status as well as enhanced the resistance of rainbow trout against ammonia toxicity.
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来源期刊
Annals of Animal Science
Annals of Animal Science 农林科学-奶制品与动物科学
CiteScore
4.00
自引率
5.30%
发文量
138
审稿时长
6-12 weeks
期刊介绍: Annals of Animal Science accepts original papers and reviews from the different topics of animal science: genetic and farm animal breeding, the biology, physiology and reproduction of animals, animal nutrition and feedstuffs, environment, hygiene and animal production technology, quality of animal origin products, economics and the organization of animal production.
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