应用Illumina HiSeq测序技术开发Fejervaria multistata富含AT的微卫星位点

IF 0.6 4区生物学 Q4 ZOOLOGY

Acta Herpetologica Pub Date : 2019-12-27 DOI:10.13128/A_H-7755

Yanmei Wang, Jing-Yi Chen, Guohua Ding, Zhi-hua Lin

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引用次数: 0

摘要

在我们的研究中，共发现2561个含有微卫星基因座的序列有可能用于引物设计。此外，Illumina HiSeq测序技术在我们的Fejervarya polystata基因组DNA序列库中鉴定出了比例最高的三核苷酸重复序列和富含AT的重复序列。从浙江省丽水市3个群体中分离到18个新的多孢镰刀菌微卫星位点，对48个个体进行了基因分型。有17个位点具有多态性，每个群体的等位基因数量在2到11个之间。多态性信息含量、观察到的杂合度和预期杂合度分别为0-0.845、0-1.0和0-0.871。在连锁不平衡中没有观察到任何位点。每个群体中有一个位点（FMA294）分别偏离Hardy-Winberg平衡，并合并。这些信息丰富的微卫星位点将适用于从个体间到群体间不同规模的多孢镰刀菌的保护遗传研究。

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AT-rich microsatellite loci development for Fejervarya multistriata by Illumina HiSeq sequencing

In our study, a total of 2561 sequences that contained microsatellite loci were found potentially to be used for primer design. Furthermore, Illumina HiSeq sequencing technology identified trinucleotide repeats and AT-rich repeats with the the highest proportion in our genomic DNA sequence library of Fejervarya multistriata. Eighteen new microsatellite loci of F. multistriata were isolated and we characterize these loci genotyping 48 individuals sampled from 3 populations in Lishui City, Zhejiang Province, China. Seventeen loci were polymorphic, with the number of alleles ranging from 2 to 11 within each population. The polymorphic information content, observed and expected heterozygosity ranged 0-0.845, 0-1.0 and 0-0.871, respectively. None of the loci was observed in linkage disequilibrium. One locus (FMA294) was deviated from Hardy-Winberg equilibrium in each population separately and combined. These informative microsatellite loci will be applicable for conservation genetic studies of F. multistriata across varying scales from inter-individual to inter-population.

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来源期刊

Acta Herpetologica ZOOLOGY-

CiteScore

1.20

自引率

12.50%

发文量

审稿时长

6 months

期刊介绍： Acta Herpetologica, a journal open to academics all over the world, offers itself as a new site for the presentation and discussion of the most recent results in the field of research on Amphibians and Reptiles, both living and extinct. The official journal of the Societas Herpetologica Italica (S.H.I.), Acta Herpetologica publishes original works – extended articles, short notes and book reviews – mostly in English, dealing with the biology and diversity of Amphibians and Reptiles.