大肠杆菌蛋白表达载体综述

Paul D. Riggs
{"title":"大肠杆菌蛋白表达载体综述","authors":"Paul D. Riggs","doi":"10.1002/cpet.23","DOIUrl":null,"url":null,"abstract":"<p><i>Escherichia</i> <i>coli</i> is often the first host chosen for recombinant protein production, due to its low cost, ease of use, and the wide range of molecular tools available. After the gene of interest is cloned, the first task of the researcher is to choose a suitable protein expression vector for protein production. The number of possible vectors is vast, but a good background of the advantages and disadvantages of the various types of vectors can help in designing a successful strategy. This unit provides an introduction to the elements that make up an expression vector and guidance in applying those elements to the production of a particular protein. © 2018 by John Wiley &amp; Sons, Inc.</p>","PeriodicalId":500994,"journal":{"name":"Current Protocols Essential Laboratory Techniques","volume":"17 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2018-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/cpet.23","citationCount":"6","resultStr":"{\"title\":\"Overview of Protein Expression Vectors for E. coli\",\"authors\":\"Paul D. Riggs\",\"doi\":\"10.1002/cpet.23\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><i>Escherichia</i> <i>coli</i> is often the first host chosen for recombinant protein production, due to its low cost, ease of use, and the wide range of molecular tools available. After the gene of interest is cloned, the first task of the researcher is to choose a suitable protein expression vector for protein production. The number of possible vectors is vast, but a good background of the advantages and disadvantages of the various types of vectors can help in designing a successful strategy. This unit provides an introduction to the elements that make up an expression vector and guidance in applying those elements to the production of a particular protein. © 2018 by John Wiley &amp; Sons, Inc.</p>\",\"PeriodicalId\":500994,\"journal\":{\"name\":\"Current Protocols Essential Laboratory Techniques\",\"volume\":\"17 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2018-08-21\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1002/cpet.23\",\"citationCount\":\"6\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Current Protocols Essential Laboratory Techniques\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/cpet.23\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current Protocols Essential Laboratory Techniques","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/cpet.23","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 6

摘要

大肠杆菌通常是重组蛋白生产的首选宿主,因为它成本低,易于使用,并且有广泛的分子工具可用。克隆目标基因后,研究人员的第一个任务是选择合适的蛋白表达载体进行蛋白生产。可能的向量的数量是巨大的,但是对各种类型向量的优点和缺点的良好背景可以帮助设计一个成功的策略。本单元介绍了组成表达载体的元素,并指导将这些元素应用于特定蛋白质的生产。©2018 by John Wiley & Sons, Inc。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Overview of Protein Expression Vectors for E. coli

Escherichia coli is often the first host chosen for recombinant protein production, due to its low cost, ease of use, and the wide range of molecular tools available. After the gene of interest is cloned, the first task of the researcher is to choose a suitable protein expression vector for protein production. The number of possible vectors is vast, but a good background of the advantages and disadvantages of the various types of vectors can help in designing a successful strategy. This unit provides an introduction to the elements that make up an expression vector and guidance in applying those elements to the production of a particular protein. © 2018 by John Wiley & Sons, Inc.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信