远近针对缺血性脑卒中大鼠神经功能及凋亡相关蛋白Bax/Bcl-2/裂解胱天蛋白酶-3表达的影响

IF 0.2 4区 医学 Q4 INTEGRATIVE & COMPLEMENTARY MEDICINE
W. Jian, Z. Can, Yang Jun, Xin Liwei, Zhang Kun, Tai Xiantao
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The sham electroacupuncture group was the same as the electroacupuncture group in acupoint electroacupuncture group was the same as the\n electroacupuncture group in acupoint selection. The electroacupuncture group was only inserted subcutaneously and then connected with the electroacupuncture group without power supply, and fixed for 30 minutes. The electroacupuncture group and the acupuncture group received electroacupuncture\n treatment once, for a total of 14 days, 1d after modeling. The Improved Neurological Impairment scale (mNSS) was used to evaluate the degree of neurological impairment in each group after anesthesia and wakefulness. The percentage of cerebral infarction area was determined by TTC staining.\n HE staining and Nissl staining were used to observe the pathological changes of ischemic brain tissue. The level of apoptosis in ischemic brain tissue was detected by TUNEL assay. 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引用次数: 1

摘要

目的探讨电针对缺血性脑卒中大鼠神经功能的影响及其抗细胞凋亡机制。方法将80只SPF雄性SD大鼠随机分为假手术组、模型组、假电针组、针刺组、电针组,每组16只。采用缝线栓塞法制备大鼠左缺血性脑卒中模型。假手术组仅分离左侧颈总动脉,不给予其他治疗。电针组选用“百会”和“命门”进行针刺,然后用扩张波,频率2Hz/100Hz,强度1mA,电针30min。假电针组与电针组在选穴方面相同。电针组仅皮下插入,然后在没有电源的情况下与电针组连接,固定30分钟。电针组和针刺组在造模后1d电针治疗1次,共14天。采用改良神经损伤量表(mNSS)评估各组麻醉和清醒后的神经损伤程度。TTC染色法测定脑梗死面积百分比。HE染色和Nissl染色观察缺血脑组织的病理变化。TUNEL法检测缺血脑组织细胞凋亡水平。Western blot检测缺血脑组织中Bax、Bcl-2和裂解的胱天蛋白酶-3的蛋白表达。结果与假手术组相比,模型组神经功能评分、脑梗死面积百分比和细胞凋亡水平均显著升高(均P<0.01),与模型组相比,针刺组和电针组缺血性脑卒中大鼠脑组织Bcl-2蛋白表达水平均不同程度上调,而电针组Bax和Cleaved胱天蛋白酶-3蛋白的表达水平下调。结论电针可抑制Bax、裂解胱天蛋白酶-3,上调Bcl-2对神经元凋亡的表达,从而改善缺血性脑卒中大鼠的神经功能损伤。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effects of near-far acupuncture on neuronal function and expression of apoptosis-related protein Bax/Bcl-2/Cleaved caspase-3 in rats with ischemic stroke
Objectives To explore the effects of electroacupuncture on nerve function in rats with ischemic stroke and its mechanism of anti-apoptosis. Methods A total of 80 SPF male SD rats were randomly divided into sham operation group, model group, sham electroacupuncture group, acupuncture group, electroacupuncture group and 16 rats in each group. The rat model of left ischemic stroke was prepared by suture embolization. In the sham group, the left common carotid artery was isolated only and no other treatment was given. In the electroacupuncture group, "Baihui" and "Mingmen" were selected for acupuncture, followed by dilatation wave, frequency 2Hz/100Hz, intensity 1mA, and electroacupuncture for 30min. The sham electroacupuncture group was the same as the electroacupuncture group in acupoint electroacupuncture group was the same as the electroacupuncture group in acupoint selection. The electroacupuncture group was only inserted subcutaneously and then connected with the electroacupuncture group without power supply, and fixed for 30 minutes. The electroacupuncture group and the acupuncture group received electroacupuncture treatment once, for a total of 14 days, 1d after modeling. The Improved Neurological Impairment scale (mNSS) was used to evaluate the degree of neurological impairment in each group after anesthesia and wakefulness. The percentage of cerebral infarction area was determined by TTC staining. HE staining and Nissl staining were used to observe the pathological changes of ischemic brain tissue. The level of apoptosis in ischemic brain tissue was detected by TUNEL assay. Western blot was used to detect protein expression of Bax, Bcl-2 and Cleaved caspase-3 in ischemic brain tissue. Results Compared with the sham operation group, neurological function score, percentage of cerebral infarction area and apoptosis level in the model group were significantly increased (all P < 0.01). Compared with the model group, neurological function score, percentage of cerebral infarction area and apoptosis index of acupuncture group and electroacupuncture group were decreased (all P <0.05). Compared with the model group, the expression levels of Bcl-2 protein in ischemic brain tissue of rats with ischemic stroke were up-regulated in the acupuncture group and electroacupuncture group to different degrees, while the expression levels of Bax and Cleaved caspase-3 protein were down-regulated in the electroacupuncture group. Conclusion Electroacupuncture may inhibit Bax, Cleaved caspase-3 and up-regulate the expression of Bcl-2 against neuronal apoptosis, thereby improving the neurological function injury of ischemic stroke rats.
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来源期刊
Acupuncture & Electro-Therapeutics Research
Acupuncture & Electro-Therapeutics Research 医学-全科医学与补充医学
CiteScore
0.50
自引率
66.70%
发文量
9
审稿时长
>12 weeks
期刊介绍: The aim of the journal is to provide an international forum for the exchange of ideas and promotion of basic and clinical research in acupuncture, electro-therapeutics, and related fields. The journal was established in order to make acupuncture and electro-therapeutics a universally acceptable branch of medicine through multidisciplinary research based on scientific disciplines. The final goal is to provide a better understanding of both the beneficial and adverse effects of these treatments in order to supplement or improve existing methods of diagnosis, prognosis, treatment, and prevention of diseases in both Western and Oriental medicine.
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