水蒸馏法提取亚麻籽精油的植物化学成分诱导A549肺癌细胞凋亡和抑制细胞迁移

IF 1.2 Q4 PHARMACOLOGY & PHARMACY

Journal of Herbs, Spices and Medicinal Plants Pub Date : 2023-01-02 DOI:10.1080/10496475.2022.2086656

Amal Dhivahar S, Berlin Grace V M, David Wilson D, S. S.

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引用次数: 0

摘要

摘要分析了亚麻仁挥发油对A549肺癌癌症细胞的抗癌作用。利用傅立叶变换红外光谱（FTIR）和气相色谱-质谱（GC-MS）对植物化学物质进行了分析。使用台盼蓝、MTT、克隆原性、DNA片段化和伤口划痕试验分析细胞毒性效应。鉴定出17种具有抗癌功效的化合物。细胞毒性随着浓度的增加而增加，并抑制癌症细胞的迁移。由于精油中的植物化学物质，凝胶中涂抹的DNA和与完整对照DNA相比增加的OD值也是可能的。

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The Phytochemicals of Linum usitatissimum Seed Essential Oil Extracted by Hydrodistillation Induced Apoptosis and Suppressed Cell Migration in A549 Lung Cancer Cells

ABSTRACT Flaxseed essential oil extracted by hydrodistillation was analyzed for its anticancer effects on A549 lung cancer cells. Phytochemicals were analyzed using Fourier-transform infrared spectroscopy (FTIR) and Gas Chromatography-Mass Spectrometry (GC-MS). The cytotoxicity effect was analyzed using Trypan blue, MTT, Clonogenic, DNA fragmentation, and wound scratch assays. Seventeen compounds with anticancer potency were identified. The cytotoxicity increased with increasing concentrations and inhibited migration of the cancer cells. Smeared DNA in gel and increased OD values compared to intact control DNA were also possible because of the phytochemicals in the essential oils.

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来源期刊

Journal of Herbs, Spices and Medicinal Plants Medicine-Complementary and Alternative Medicine

CiteScore

3.00

自引率

0.00%

发文量

期刊介绍： The Journal of Herbs, Spices & Medicinal Plants is an essential reference filled with recent research and other valuable information associated with herbs, spices, and medicinal plants. The Journal serves as a focus point through which investigators and others may publish material of importance to the production, marketing, and utilization of these plants and associated extracts. The journal covers the following topics: growth, development, horticulture, ecology, physiology, genetics, chemistry, and economics. Original articles, review articles, and book reviews provide information of interest to an international audience of researchers, teachers, technicians, and managers involved with production and/or marketing of herbs, spices, and medicinal plants. Managers of food companies, food processing facilities, medical research laboratories, government agencies, and others interested in new chemicals, food additives, international trade, patents, and other items can easily review new findings. The Journal of Herbs, Spices & Medicinal Plants is a forum in which recent research and other information associated with herbs, spices, and medicinal plants is shared. The Journal represents a centralized database accessible by investigators within the international community that work with or have an interest in herbs, spices, and medicinal plants.