原子力显微镜和膜片钳分析不同尺寸1,2-二醇基-sn-3-磷酸胆碱脂质体的单板层特性

IF 0.1 4区 医学
Chi Hyung Lee, J. Kwak, S. Sung, Sang Weon Lee, J. Kwak, Kyung-wan Kim, H. Kang, G. Kim
{"title":"原子力显微镜和膜片钳分析不同尺寸1,2-二醇基-sn-3-磷酸胆碱脂质体的单板层特性","authors":"Chi Hyung Lee, J. Kwak, S. Sung, Sang Weon Lee, J. Kwak, Kyung-wan Kim, H. Kang, G. Kim","doi":"10.1166/jbt.2023.3263","DOIUrl":null,"url":null,"abstract":"DOPC liposome properties of various sizes were analyzed via atomic force microscopy (AFM) and patch-clamp. The unilamellarity of small DOPC liposome (below 1 μm) was confirmed via Cryogenic transmission electron microscopy (Cryo-TEM). Small DOPC liposome (below 1 μm)\n showed a bending modulus (kbend) ranging (between 10−18 and 10−20 J). The bending modulus value was a size dependence. In order words, it decreased as DOPC liposome size increased. For DOPC liposome (above 1 μm), patch-clamp was\n used to achieve electrically tight whole-cell configurations. Our result showed that the unilamellar DOPC liposome (above 1 μm) exhibit RC circuit response property. It was similar to theoretical values (τ = 4.52 ms) of the unilamellar liposome. In this study, the uniform\n lamellarity of the DOPC liposome of various sizes was confirmed through electrical and mechanical properties of DOPC liposome.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":" ","pages":""},"PeriodicalIF":0.1000,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Unilamellar Characteristic Analysis of 1,2-dioleoyl-sn-3-phosphocholine Liposome of Various Sizes via Atomic Force Microscopy and Patch-Clamp\",\"authors\":\"Chi Hyung Lee, J. Kwak, S. Sung, Sang Weon Lee, J. Kwak, Kyung-wan Kim, H. Kang, G. Kim\",\"doi\":\"10.1166/jbt.2023.3263\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"DOPC liposome properties of various sizes were analyzed via atomic force microscopy (AFM) and patch-clamp. The unilamellarity of small DOPC liposome (below 1 μm) was confirmed via Cryogenic transmission electron microscopy (Cryo-TEM). Small DOPC liposome (below 1 μm)\\n showed a bending modulus (kbend) ranging (between 10−18 and 10−20 J). The bending modulus value was a size dependence. In order words, it decreased as DOPC liposome size increased. For DOPC liposome (above 1 μm), patch-clamp was\\n used to achieve electrically tight whole-cell configurations. Our result showed that the unilamellar DOPC liposome (above 1 μm) exhibit RC circuit response property. It was similar to theoretical values (τ = 4.52 ms) of the unilamellar liposome. In this study, the uniform\\n lamellarity of the DOPC liposome of various sizes was confirmed through electrical and mechanical properties of DOPC liposome.\",\"PeriodicalId\":15300,\"journal\":{\"name\":\"Journal of Biomaterials and Tissue Engineering\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":0.1000,\"publicationDate\":\"2023-02-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Biomaterials and Tissue Engineering\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1166/jbt.2023.3263\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Biomaterials and Tissue Engineering","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1166/jbt.2023.3263","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

摘要

通过原子力显微镜(AFM)和膜片钳分析了不同尺寸DOPC脂质体的性质。通过低温透射电镜(Cryo-TEM)证实了DOPC小脂质体(小于1 μm)的单晶性。小DOPC脂质体(小于1 μm)的弯曲模量(kbend)在10−18 ~ 10−20 J之间,弯曲模量值与尺寸有关。也就是说,随着DOPC脂质体大小的增加,它降低。对于DOPC脂质体(大于1 μm),采用膜片钳实现电紧全细胞结构。结果表明,1 μm以上的单层DOPC脂质体具有RC电路响应特性。这与单层脂质体的理论值(τ = 4.52 ms)相似。在本研究中,通过DOPC脂质体的电学和力学性能,证实了不同尺寸DOPC脂质体的均匀片层性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Unilamellar Characteristic Analysis of 1,2-dioleoyl-sn-3-phosphocholine Liposome of Various Sizes via Atomic Force Microscopy and Patch-Clamp
DOPC liposome properties of various sizes were analyzed via atomic force microscopy (AFM) and patch-clamp. The unilamellarity of small DOPC liposome (below 1 μm) was confirmed via Cryogenic transmission electron microscopy (Cryo-TEM). Small DOPC liposome (below 1 μm) showed a bending modulus (kbend) ranging (between 10−18 and 10−20 J). The bending modulus value was a size dependence. In order words, it decreased as DOPC liposome size increased. For DOPC liposome (above 1 μm), patch-clamp was used to achieve electrically tight whole-cell configurations. Our result showed that the unilamellar DOPC liposome (above 1 μm) exhibit RC circuit response property. It was similar to theoretical values (τ = 4.52 ms) of the unilamellar liposome. In this study, the uniform lamellarity of the DOPC liposome of various sizes was confirmed through electrical and mechanical properties of DOPC liposome.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
332
审稿时长
>12 weeks
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信