阿尔及利亚秀丽隐杆线虫水提取物具有较强的抗溶血和抗氧化性能

Q4 Pharmacology, Toxicology and Pharmaceutics
Brahim Asseli, Reguia Mahfoudi, A. Djeridane, M. Yousfi
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引用次数: 1

摘要

对药用植物抗氧化剂的研究已经成为一种潜在的治疗方法,可以预防自由基对人体造成的损伤。Hammada elegans Botsch(俗称“Ajram”)是一种广泛存在于拉古亚特地区的旱生植物,但关于这些物种的生物或化学特性的报道很少。因此,本研究的目的是通过采用不同的体外测定系统,研究阿尔及利亚秀丽隐杆线虫地上部分的己烷、丙酮、甲醇和水提取物的抗氧化和抗溶血活性。分别用Folin-Ciocalteu、氯化铝和香草醛测定法分析了总酚含量、黄酮含量和缩合单宁含量。提取物的体外抗氧化能力通过CURAC、铁螯合、ABTS•+和抗溶血测定进行评估,并表示为EC50值。在所分析的提取物中,水提取物的酚类、黄酮类和单宁含量最高。此外,与其他提取物和标准品相比,该提取物显示出最高的抗氧化能力。其对ABTS自由基清除活性的EC50值为0.265±0.003mg/L。此外,该提取物显示出高的铁(II)螯合能力(EC50=0.958±0.001mg/L),并且在铜离子还原活性(CURAC)中具有良好的抗氧化活性(EC50为0.709±0.002mg/L),且呈浓度依赖性。此外,该提取物对AAPH诱导的溶血具有最好的抗溶血活性(EC50=0.090±0.004mg/L)。我们的研究表明,秀丽隐杆线虫的水提取物是一种潜在的抗氧化剂来源,具有很高的膜抗自由基保护作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Strong antihemolytic and antioxidant properties of aqueous extract from Algerian Hammada elegans (Bge.) Botsch (Chenopodiaceae)
Research on medicinal plant antioxidants has emerged as a potential therapeutic to prevent free radical generated damage in the human body. Hammada elegans Botsch (popularly known as “Ajram”) is a xerophytic plant widely found in Laghouat region, but there are only a few reports about the biological or chemical properties of these species. Hence, the aim of this study is to investigate the antioxidant and the antihemolytic activities of hexanic, acetonic, methanolic and aqueous extracts of aerial parts of Algerian Hammada elegans Botsch by employing different in vitro assay systems. The total phenolic content, the flavonoid content and the condensed tannin amount were analyzed using Folin-Ciocalteu, aluminum chloride and vanillin assays, respectively. The in vitro antioxidant capacity of extracts was assessed by CUPRAC, iron chelating, ABTS•+and antihemolytic assays, and was expressed as EC50 values. Among the analyzed extracts, the aqueous extract had the highest phenolic, flavonoid and tannin contents. Also, this extract displayed the highest antioxidant capacities compared to the other extracts and standards. Its EC50 value for ABTS radical-scavenging activity was 0.265 ± 0.003 mg/L. Moreover, this extract showed high iron (II) chelating ability (EC50 = 0.958 ± 0.001 mg/L), and good antioxidant activity in the cupric ion reducing activity (CUPRAC) in a concentration dependent manner (EC50 were 0.709 ± 0.002 mg/L). Additionally, this extract had the best antihemolytic activity against AAPH-induced hemolysis (EC50=0.090 ± 0.004 mg/L). Our study revealed that the aqueous extract of Hammada elegans Botsch, is a potential source of antioxidants which possess a high protective effect of membrane against free radical.
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来源期刊
Current Enzyme Inhibition
Current Enzyme Inhibition Pharmacology, Toxicology and Pharmaceutics-Drug Discovery
CiteScore
1.30
自引率
0.00%
发文量
30
期刊介绍: Current Enzyme Inhibition aims to publish all the latest and outstanding developments in enzyme inhibition studies with regards to the mechanisms of inhibitory processes of enzymes, recognition of active sites, and the discovery of agonists and antagonists, leading to the design and development of new drugs of significant therapeutic value. Each issue contains a series of timely, in-depth reviews written by leaders in the field, covering a range of enzymes that can be exploited for drug development. Current Enzyme Inhibition is an essential journal for every pharmaceutical and medicinal chemist who wishes to have up-to-date knowledge about each and every development in the study of enzyme inhibition.
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