百虎加人参汤UHPLC-Q /Orbitrap/MS/MS指纹图谱及对链脲霉素诱导的糖尿病大鼠抗氧化活性的评价

IF 1.7 4区化学 Q3 CHEMISTRY, ANALYTICAL

Acta Chromatographica Pub Date : 2022-10-10 DOI:10.1556/1326.2022.01068

Libin Wang, Xin Shen, Fang Wang, Xiaohui Xu

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引用次数: 0

摘要

白虎夹肾汤是治疗糖尿病最古老的经典中药方剂之一。然而，据我们所知，该处方的成分尚未确定，关于该处方抗糖尿病机制的研究也很少。因此，BHJRSTD是通过超高效液相色谱联用四极杆Exactive Focus Orbitrap MS（UHPLC–Q/Orbitrap/MS/MS）进行检测和鉴定的。我们鉴定了74种化合物，包括黄酮类、生物碱、查尔酮、黄酮、酚类、苯丙烷类、萜类、三萜类、氨基酸衍生物等。然后，Sprague-Dawley大鼠喂食高脂高糖饮食两个月，并注射链脲佐菌素（STZ）诱导2型糖尿病（T2DM）。糖尿病大鼠随机接受二甲双胍（200 mg kg−1·d−1，n=15），BHJRSTD提取物（40 g kg−1·d−1）和BHJRSTD提取物（10 g kg−1·d−1）灌胃8周。结果证实，BHJRSTD能显著降低MDA水平，提高过氧化氢酶（CAT）、超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）水平，表明该方对治疗T2DM具有显著的抗氧化活性。

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UHPLC–Q/Orbitrap/MS/MS fingerprinting of Bai-Hu-Jia-Ren-Shen-Tang Decoction and evaluation of its antioxidant activity in streptozotocin-induced diabetic rats

Bai-Hu-Jia-Ren-Shen-Tang Decoction (BHJRSTD) is one of the oldest classic Chinese medicine prescriptions which used in the field of treatment of diabetes. However, to the best of our knowledge, the ingredients of this prescription have not been identified, and there are very few studies on the anti-diabetic mechanism of this prescription. Therefore, BHJRSTD was detected and identified by ultra-high-performance liquid chromatography coupled with Quadrupole-Exactive Focus Orbitrap MS (UHPLC–Q/Orbitrap/MS/MS). We identified 74 compounds, including flavonoids, alkaloids, chalcones, xanthones, phenols, phenylpropanoids, terpenes, triterpenes, amino acid derivatives, etc. Then, Sprague Dawley rats were fed with a high-fat and high-sugar diet for two months and injected with streptozotocin (STZ) to induce type 2 diabetes (T2DM). The diabetic rats were randomized to given metformin (200 mg kg−1·d−1, n = 15), BHJRSTD extracts (40 g kg−1·d−1) and BHJRSTD extracts (10 g kg−1·d−1) by gavage for 8 weeks. The results confirmed that BHJRSTD significantly decreased the level of MDA and increased levels of catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), it shows that the prescription has significant antioxidant activity in the treatment of T2DM.

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来源期刊

Acta Chromatographica 化学-分析化学

CiteScore

4.00

自引率

0.00%

发文量

审稿时长

2.3 months

期刊介绍： Acta Chromatographica Open Access Acta Chromatographica publishes peer-reviewed scientific articles on every field of chromatography, including theory of chromatography; progress in synthesis and characterization of new stationary phases; chromatography of organic, inorganic and complex compounds; enantioseparation and chromatography of chiral compounds; applications of chromatography in biology, pharmacy, medicine, and food analysis; environmental applications of chromatography; analytical and physico-chemical aspects of sample preparation for chromatography; hyphenated and combined techniques; chemometrics and its applications in separation science.