接受高热量饮食的不同品系大鼠纹状体中多巴胺、5-羟色胺及其代谢物的水平

Q3 Agricultural and Biological Sciences

Biological Communications Pub Date : 2021-12-30 DOI:10.21638/spbu03.2021.405

S. Apryatin, Evgenia Efimova, Z. Fesenko, A. Shumakova, I. Gmoshinski

{"title":"接受高热量饮食的不同品系大鼠纹状体中多巴胺、5-羟色胺及其代谢物的水平","authors":"S. Apryatin, Evgenia Efimova, Z. Fesenko, A. Shumakova, I. Gmoshinski","doi":"10.21638/spbu03.2021.405","DOIUrl":null,"url":null,"abstract":"The aim of this work was to study the effect of a high fat and carbohydrate diet (HFCD) and quercetin supplementation on the levels of dopamine (DA), serotonin (5-HT) and their metabolites in Wistar, DA transporter knockout (DAT-KO) and obese Zucker fa/fa rats. Animals received a control diet or HFCD for 62 days. Wistar and Zucker fa/fa rats received quercetin. The contents of DA, 5-HT, norepinephrine (NE), dioxyphenyl acetate (DOPAC), homovanillic acid (HVA) and 5-hydroxyindolyl acetate (5-HIIA) in the striatum were determined by high-performance liquid chromatography (HPLC). DAT-KO homozygotes had lowered DA and increased HVA and DOPAC compared to Wistar rats. HFCD did not affect the content of NE and 5-HT. 5-HT was increased in DAT-KO homozygotes compared with Wistar receiving a control diet. 5-HIIA accumulated in larger amounts in DAT-KO compared to Wistar with the exception of those receiving quercetin with a control diet. Quercetin did not affect the levels of DA, 5-HT and their metabolites.","PeriodicalId":8998,"journal":{"name":"Biological Communications","volume":" ","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2021-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Levels of dopamine, 5-hydroxytryptamine and their metabolites in the striatum of rats of various strains receiving a high-calorie diet\",\"authors\":\"S. Apryatin, Evgenia Efimova, Z. Fesenko, A. Shumakova, I. Gmoshinski\",\"doi\":\"10.21638/spbu03.2021.405\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The aim of this work was to study the effect of a high fat and carbohydrate diet (HFCD) and quercetin supplementation on the levels of dopamine (DA), serotonin (5-HT) and their metabolites in Wistar, DA transporter knockout (DAT-KO) and obese Zucker fa/fa rats. Animals received a control diet or HFCD for 62 days. Wistar and Zucker fa/fa rats received quercetin. The contents of DA, 5-HT, norepinephrine (NE), dioxyphenyl acetate (DOPAC), homovanillic acid (HVA) and 5-hydroxyindolyl acetate (5-HIIA) in the striatum were determined by high-performance liquid chromatography (HPLC). DAT-KO homozygotes had lowered DA and increased HVA and DOPAC compared to Wistar rats. HFCD did not affect the content of NE and 5-HT. 5-HT was increased in DAT-KO homozygotes compared with Wistar receiving a control diet. 5-HIIA accumulated in larger amounts in DAT-KO compared to Wistar with the exception of those receiving quercetin with a control diet. Quercetin did not affect the levels of DA, 5-HT and their metabolites.\",\"PeriodicalId\":8998,\"journal\":{\"name\":\"Biological Communications\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2021-12-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biological Communications\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.21638/spbu03.2021.405\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"Agricultural and Biological Sciences\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biological Communications","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.21638/spbu03.2021.405","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}

引用次数: 0

摘要

本工作的目的是研究高脂肪和碳水化合物饮食（HFCD）和槲皮素补充对Wistar、DA转运蛋白敲除（DAT-KO）和肥胖Zucker fa/fa大鼠多巴胺（DA）、5-羟色胺（5-HT）及其代谢产物水平的影响。动物接受对照饮食或HFCD治疗62天。Wistar和Zucker fa/fa大鼠接受槲皮素。采用高效液相色谱法测定了纹状体中DA、5-HT、去甲肾上腺素（NE）、乙酸二氧苯基酯（DOPAC）、高香草酸（HVA）和5-羟基吲哚乙酸（5-HIIA）的含量。与Wistar大鼠相比，DAT-KO纯合子降低了DA并增加了HVA和DOPAC。HFCD不影响NE和5-HT的含量。与接受对照饮食的Wistar相比，DAT-KO纯合子中的5-HT增加。与Wistar相比，5-HIIA在DAT-KO中积累的量更大，除了那些用对照饮食接受槲皮素的人。槲皮素不影响DA、5-HT及其代谢产物的水平。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Levels of dopamine, 5-hydroxytryptamine and their metabolites in the striatum of rats of various strains receiving a high-calorie diet

The aim of this work was to study the effect of a high fat and carbohydrate diet (HFCD) and quercetin supplementation on the levels of dopamine (DA), serotonin (5-HT) and their metabolites in Wistar, DA transporter knockout (DAT-KO) and obese Zucker fa/fa rats. Animals received a control diet or HFCD for 62 days. Wistar and Zucker fa/fa rats received quercetin. The contents of DA, 5-HT, norepinephrine (NE), dioxyphenyl acetate (DOPAC), homovanillic acid (HVA) and 5-hydroxyindolyl acetate (5-HIIA) in the striatum were determined by high-performance liquid chromatography (HPLC). DAT-KO homozygotes had lowered DA and increased HVA and DOPAC compared to Wistar rats. HFCD did not affect the content of NE and 5-HT. 5-HT was increased in DAT-KO homozygotes compared with Wistar receiving a control diet. 5-HIIA accumulated in larger amounts in DAT-KO compared to Wistar with the exception of those receiving quercetin with a control diet. Quercetin did not affect the levels of DA, 5-HT and their metabolites.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Biological Communications Agricultural and Biological Sciences-Agricultural and Biological Sciences (all)

CiteScore

1.70

自引率

0.00%

发文量