绿僵菌生防菌CBS 648.67和CFCC 80795胞内新型细菌的检测与鉴定

IF 2.1 4区环境科学与生态学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Microbes and Environments Pub Date : 2022-05-24 DOI:10.1264/jsme2.ME21059

Yue Ying, Chenglin Liu, Ran He, Ruizhen Wang, Liangjian Qu

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引用次数: 0

摘要

“内共生”是一种共生关系的内聚形式。内细菌存在于许多真菌中，在真菌宿主生物学中起着重要作用。绿僵菌属是防治害虫的重要昆虫病原真菌。在本研究中，我们利用PCR、系统发育和荧光电镜对bibionidarum Metarhizium bibionidum和M. anisopliae菌株进行了综合分析，以鉴定菌丝和分生孢子内的内细菌。基于16S rRNA基因序列的系统发育分析结果表明，这些内菌与puraquae Pelomonas亲缘关系最密切，隶属于Betaproteobacteria。超微结构观察表明，内细菌呈球粒状，直径小于500 nm。阐明了bibionidarum和m.s apilpliae内细菌的基本特征，并对其在绿僵菌宿主中的生物学功能提出了疑问。

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Detection and Identification of Novel Intracellular Bacteria Hosted in Strains CBS 648.67 and CFCC 80795 of Biocontrol Fungi Metarhizium

“Endosymbiosis” is a cohesive form of a symbiotic association. Endobacteria exist in many fungi and play important roles in fungal host biology. Metarhizium spp. are important entomopathogenic fungi for insect pest control. In the present study, we performed comprehensive analyses of strains of Metarhizium bibionidarum and M. anisopliae using PCR, phylogenetics, and fluorescent electron microscopy to identify endobacteria within hyphae and conidia. The results of the phylogenetic analysis based on 16S rRNA gene sequences indicated that these endobacteria were the most closely related to Pelomonas puraquae and affiliated with Betaproteobacteria. Ultrastructural observations indicated that endobacteria were coccoid and less than 500 nm in diameter. The basic characteristics of endobacteria in M. bibionidarum and M. anisopliae were elucidated, and biological questions were raised regarding their biological functions in the Metarhizium hosts.

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来源期刊

Microbes and Environments 生物-生物工程与应用微生物

CiteScore

4.10

自引率

13.60%

发文量

审稿时长

3 months

期刊介绍： Microbial ecology in natural and engineered environments; Microbial degradation of xenobiotic compounds; Microbial processes in biogeochemical cycles; Microbial interactions and signaling with animals and plants; Interactions among microorganisms; Microorganisms related to public health; Phylogenetic and functional diversity of microbial communities; Genomics, metagenomics, and bioinformatics for microbiology; Application of microorganisms to agriculture, fishery, and industry; Molecular biology and biochemistry related to environmental microbiology; Methodology in general and environmental microbiology; Interdisciplinary research areas for microbial ecology (e.g., Astrobiology, and Origins of Life); Taxonomic description of novel microorganisms with ecological perspective; Physiology and metabolisms of microorganisms; Evolution of genes and microorganisms; Genome report of microorganisms with ecological perspective.