多年生植物在绵羊胃肠道线虫防治中的应用

A. Bosco, A. Prigioniero, Alessandra Falzarano, M. P. Maurelli, L. Rinaldi, G. Cringoli, G. Quaranta, S. Claps, R. Sciarrillo, C. Guarino, P. Scarano
{"title":"多年生植物在绵羊胃肠道线虫防治中的应用","authors":"A. Bosco, A. Prigioniero, Alessandra Falzarano, M. P. Maurelli, L. Rinaldi, G. Cringoli, G. Quaranta, S. Claps, R. Sciarrillo, C. Guarino, P. Scarano","doi":"10.3389/fpara.2023.1186149","DOIUrl":null,"url":null,"abstract":"Background Gastrointestinal nematodes (GINs) are a serious problem in ruminant pastures worldwide. They generate production losses, from the point of view of both the food chain and animal mortality. This study provides preliminary results concerning the use of pasture plants in the Campania region (of southern Italy) to control GINs in sheep. Methods Sixteen species of wild and cultivated perennial plants present in seminatural pastures were sampled. All species were extracted with the conventional maceration technique, using three different solvents (i.e., H2O, EtOH, and an EtOH:H2O (8:2) mixture) in order to extract different bioactive compounds. The total phenolic content (TPC; assessed via the Folin–Ciocȃlteu assay) of all extract samples was preliminarily characterized. Each sample was aliquoted across six different concentrations and an in vitro egg hatching test (EHT) was conducted to evaluate the ovicidal effect on sheep GINs. Results The results indicated that Cichorium intybus L. and Foeniculum vulgare Mill. extracts greatly inhibited egg hatching within 48 hours of exposure, showing efficacy (≥ 62.6%) at the three higher concentrations when compared with the other plants. Conclusion The use of extracts of wild and cultivated perennial plant species in pastures can be a valid alternative to the use of synthetic anthelmintic drugs, which can generate problems, such as anthelmintic resistance, in the long term. Looking forward, further in vitro studies that evaluate the in vitro effect of these extracts on ruminant cell cultures, and field application through in vivo studies, would likely confirm the results obtained from preliminary in vitro EHTs. All these studies should be aimed at evaluating the therapeutic potential and future applicability of specific plant cultures in pastures to achieve sustainable pest control.","PeriodicalId":73098,"journal":{"name":"Frontiers in parasitology","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2023-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Use of perennial plants in the fight against gastrointestinal nematodes of sheep\",\"authors\":\"A. Bosco, A. Prigioniero, Alessandra Falzarano, M. P. Maurelli, L. Rinaldi, G. Cringoli, G. Quaranta, S. Claps, R. Sciarrillo, C. Guarino, P. Scarano\",\"doi\":\"10.3389/fpara.2023.1186149\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Background Gastrointestinal nematodes (GINs) are a serious problem in ruminant pastures worldwide. They generate production losses, from the point of view of both the food chain and animal mortality. This study provides preliminary results concerning the use of pasture plants in the Campania region (of southern Italy) to control GINs in sheep. Methods Sixteen species of wild and cultivated perennial plants present in seminatural pastures were sampled. All species were extracted with the conventional maceration technique, using three different solvents (i.e., H2O, EtOH, and an EtOH:H2O (8:2) mixture) in order to extract different bioactive compounds. The total phenolic content (TPC; assessed via the Folin–Ciocȃlteu assay) of all extract samples was preliminarily characterized. Each sample was aliquoted across six different concentrations and an in vitro egg hatching test (EHT) was conducted to evaluate the ovicidal effect on sheep GINs. Results The results indicated that Cichorium intybus L. and Foeniculum vulgare Mill. extracts greatly inhibited egg hatching within 48 hours of exposure, showing efficacy (≥ 62.6%) at the three higher concentrations when compared with the other plants. Conclusion The use of extracts of wild and cultivated perennial plant species in pastures can be a valid alternative to the use of synthetic anthelmintic drugs, which can generate problems, such as anthelmintic resistance, in the long term. Looking forward, further in vitro studies that evaluate the in vitro effect of these extracts on ruminant cell cultures, and field application through in vivo studies, would likely confirm the results obtained from preliminary in vitro EHTs. All these studies should be aimed at evaluating the therapeutic potential and future applicability of specific plant cultures in pastures to achieve sustainable pest control.\",\"PeriodicalId\":73098,\"journal\":{\"name\":\"Frontiers in parasitology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-06-02\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Frontiers in parasitology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.3389/fpara.2023.1186149\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Frontiers in parasitology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3389/fpara.2023.1186149","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

摘要

背景胃肠道线虫(GINs)是世界范围内反刍动物牧场的一个严重问题。从食物链和动物死亡率的角度来看,它们造成了生产损失。本研究提供了关于在坎帕尼亚地区(意大利南部)使用牧场植物控制绵羊GINs的初步结果。方法对半天然牧场的16种多年生野生和栽培植物进行取样。所有物种都用传统的浸渍技术提取,使用三种不同的溶剂(即H2O、EtOH和EtOH:H2O(8:2)混合物),以提取不同的生物活性化合物。初步表征了所有提取物样品的总酚含量(TPC;通过Folin–Ciocȃlteu测定法评估)。每个样本在六种不同浓度下等分,并进行体外卵孵化试验(EHT)以评估对绵羊GINs的杀卵作用。结果表明菊苣和小茴香具有较好的药用价值。提取物在暴露48小时内显著抑制卵孵化,与其他植物相比,在三个较高浓度下显示出有效性(≥62.6%)。结论在牧场使用野生和栽培多年生植物的提取物可以有效地替代使用合成驱虫药,从长远来看,合成驱虫药会产生抗药性等问题。展望未来,进一步的体外研究,评估这些提取物对反刍动物细胞培养的体外作用,并通过体内研究进行现场应用,可能会证实初步体外EHT的结果。所有这些研究都应旨在评估牧场中特定植物培养物的治疗潜力和未来适用性,以实现可持续的害虫控制。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Use of perennial plants in the fight against gastrointestinal nematodes of sheep
Background Gastrointestinal nematodes (GINs) are a serious problem in ruminant pastures worldwide. They generate production losses, from the point of view of both the food chain and animal mortality. This study provides preliminary results concerning the use of pasture plants in the Campania region (of southern Italy) to control GINs in sheep. Methods Sixteen species of wild and cultivated perennial plants present in seminatural pastures were sampled. All species were extracted with the conventional maceration technique, using three different solvents (i.e., H2O, EtOH, and an EtOH:H2O (8:2) mixture) in order to extract different bioactive compounds. The total phenolic content (TPC; assessed via the Folin–Ciocȃlteu assay) of all extract samples was preliminarily characterized. Each sample was aliquoted across six different concentrations and an in vitro egg hatching test (EHT) was conducted to evaluate the ovicidal effect on sheep GINs. Results The results indicated that Cichorium intybus L. and Foeniculum vulgare Mill. extracts greatly inhibited egg hatching within 48 hours of exposure, showing efficacy (≥ 62.6%) at the three higher concentrations when compared with the other plants. Conclusion The use of extracts of wild and cultivated perennial plant species in pastures can be a valid alternative to the use of synthetic anthelmintic drugs, which can generate problems, such as anthelmintic resistance, in the long term. Looking forward, further in vitro studies that evaluate the in vitro effect of these extracts on ruminant cell cultures, and field application through in vivo studies, would likely confirm the results obtained from preliminary in vitro EHTs. All these studies should be aimed at evaluating the therapeutic potential and future applicability of specific plant cultures in pastures to achieve sustainable pest control.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信