梨对干旱胁迫的响应及AVP1基因的作用

IF 0.7 4区生物学 Q4 PLANT SCIENCES

American Fern Journal Pub Date : 2023-03-17 DOI:10.1640/0002-8444-113.1.14

Jingwen Liang, Junliang Yang, Zihan Yev, Zeping Cai, Shitao Xu, Peng Wang, Xudong Yu

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引用次数: 0

摘要

摘要为了研究野穗子对干旱胁迫的响应，并寻找其V-PPase (AVP1)同源基因，进行了温室试验。我们测定了干旱处理后的一系列生理生化指标，并利用RT-qPCR分析了AVP1同源基因在野刺草中的表达。结果表明，刺槐通过限制气孔导度来适应干旱，从而抑制植物的光合作用，降低植物的生理活性。此外，野刺草还可以通过增加脯氨酸含量来改变其渗透电位，维持正常的代谢活动，并通过增加超氧化物歧化酶(SOD)和过氧化物酶(POD)的活性来防止活性氧的破坏。根据基因的相对表达量分析，AVP1-2和AVP1-4可能是野针茅的抗旱基因。本研究为进一步探索野刺草的耐旱机理和抗旱基因奠定了基础。

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Response of Asplenium nidus to Drought Stress and Roles of AVP1 Genes

Abstract. To explore how Asplenium nidus responds to drought stress and to find V-PPase (AVP1) homologous genes of this species, we conducted greenhouse experiments. We measured a series of physiological and biochemical indices after drought treatment and analyzed the expression of AVP1 homologous genes in A. nidus by RT-qPCR. The results indicated that A. nidus can adapt to drought by limiting stomatal conductance, thus inhibiting plant photosynthesis and reducing physiological activities. In addition, A. nidus can also change its osmotic potential by increasing proline content to maintain normal metabolic activities, and prevent the destruction of reactive oxygen species by increasing the activities of superoxide dismutase (SOD) and peroxidase (POD). According to analysis of the relative expression levels of genes, AVP1-2 and AVP1-4 may be the drought-resistant genes of A. nidus. This study lays a foundation for further exploration of the drought tolerance mechanism and drought-resistant genes of A. nidus.

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