壳聚糖抗坏血酸作为底物测定全唾液的水解活性

IF 0.8 Q4 MATERIALS SCIENCE, BIOMATERIALS

Progress on Chemistry and Application of Chitin and its Derivatives Pub Date : 2018-09-10 DOI:10.15259/PCACD.23.009

B. Kochańska, Mirela Łukaszewska, Jolanta Ochocińska

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引用次数: 0

摘要

研究了壳聚糖抗坏血酸作为底物对全唾液的水解活性。为此，测定了加入壳聚糖抗坏血酸前、加入壳聚糖抗坏血酸后和与壳聚糖抗坏血酸孵育20小时后唾液中n -乙酰- d -氨基葡萄糖的浓度。体外实验采用无菌壳聚糖制备抗坏血酸粉末。以磷虾甲壳素为原料制备壳聚糖。抗坏血酸与壳聚糖的比例为1:1。未受刺激的全唾液在壳聚糖抗坏血酸作为底物存在下表现出水解活性。

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DETERMINATION OF THE HYDROLYTIC ACTIVITY OF WHOLE SALIVA USING CHITOSAN ASCORBATE AS A SUBSTRATE

The aim of this work was to evaluate of hydrolytic activity of whole saliva using chitosan ascorbate as a substrate. In this aim, the concentrations of N-acetyl-D-glucosamine were determined in saliva before addition of chitosan ascorbate, directly after addition and during incubation with chitosan ascorbate by 20 hrs. In this in vitro study were used sterile chitosan ascorbate in the form of powder. Chitosan was obtained from krill chitin. The ratio of ascorbic acid to chitosan was 1:1. The unstimulated whole saliva showed the hydrolytic activity in the presence of the chitosan ascorbate as a substrate.

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来源期刊

Progress on Chemistry and Application of Chitin and its Derivatives MATERIALS SCIENCE, BIOMATERIALS-

CiteScore

1.10

自引率

16.70%

发文量

期刊介绍： Progress in the Chemistry and Application of Chitin and its Derivatives is an annual journal focused on all aspects of production, modification, enzymology and application of chitin and its many derivatives, including chitosan. The journal publishes full-length papers as well as invited reviews. To be considered, papers must present original research that has not been published or accepted for publication elsewhere. The language of the journal will be English.